Recent changes in the land use pattern have severely impacted wildlife, specifically large carnivores like leopards, by reducing natural habitat and prey base. Being highly adaptable, with a distribution more outside than inside the protected areas, leopard very often attacks human and livestock. In human-leopard conflicts, once an animal is declared as man-eater, it is either translocated or killed by officials as per Wildlife (Protection) Act 1972 of India. Identification of conflicting leopard is very difficult and sometimes lead to the castigation of innocent animal. Here, we describe the individualization of a leopard from one such human-leopard conflict in Bilaspur district of Himachal Pradesh, India using modern molecular genetic techniques. The methodology suggested in this study would be of great importance in correct identification of conflicting animals.
Genetic analysis is an important tool in understanding population structure, genetic diversity, and phylogenetics of endangered species likely to be affected by microevolution and anthropogenic factors. Western Ghats landscape is one of the identified biodiversity hotspots in India, and micro-evolutionary processes are observed in this landscape due to the presence of the gaps in the mountain ranges. Nilgiri tahr is endemic to and distributed in this landscape while very little is known about genetic characteristics, population structure and impact of these gaps on the species. In the present study, two different populations of Nilgiri tahr from the north (NPG) and south (SPG) of Palghat gap (PG) were studied using the cytochrome b gene (Cyt b; 310 bp) of mtDNA genome in the Western Ghats, India. Two variable sites were observed in the Cyt b fragment while the mean pairwise genetic distance between these two populations was 0.007. All the samples phylogenetically clustered in either north or south of PG. The presence of shallow divergence indicates the presence of suitable habitat in past which may have facilitated movement between NPG and SPG. A subsequent change in Paleo-climatic conditions and gradual formation of PG may have resulted in population diversification during the Pleistocene. Besides, Forensically Informative Nucleotide Sequence (FINS) observed would help in geoassigning any individual from NPG or SPG to understand the likely influences on population demography due to poaching.
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