Bats play key ecological roles, also hosting many zoonotic pathogens. Neotropical bat microbiota is still poorly known. We speculate that their dietary habits strongly influence their microbiota richness and antibiotic-resistance patterns, which represent growing and serious public health and environmental issue. Here we describe the aerobic microbiota richness of bats from an Atlantic Forest remnant in Southeastern Brazil, and the antibiotic-resistance patterns of bacteria of clinical importance. Oral and rectal cavities of 113 bats from Carlos Botelho State Park were swabbed. Samples were plated on 5% sheep blood and MacConkey agar and identified by the MALDI-TOF technique. Antibiotic susceptibility tests were performed using Kirby-Bauer’s antibiotic disc diffusion technique.We identified 596 isolates at the genus level and tentatively to the species level. Proteobacteria was the most abundant phylum in all the dietary guilds, representing 87% of the total identified samples. The most common bacteria within bat individuals were Escherichia coli, Klebsiella oxytoca and Serratia marcescens, and within bat species were Serratia marcescens, Pseudomonas sp. and Staphylococcus sp. Frugivores presented the most diverse microbiota. In general, the antibiogram results indicated a low occurrence of resistance on eigth potentially pathogenic bacteria species. The resistance to antibiotics found on our samples was related mostly to the intrinsic resistance of the tested species.The low occurrence of resistant bacteria in our samples could be related to the well preserved environment where bats were caught. Once the major causes of resistance-acquiring are related to anthropic activites, the controlled access of tourists on certain regions of the Park seems to be effectively protecting the environment.

Myotis nesopolus currently comprises two subspecies. The nominate subspecies (M. n. nesopolus) occurs on the Caribbean islands of Curaçao and Bonaire, Netherlands Antilles, whereas M. n. larensis is known from mainland South America in northeastern Colombia and northwestern Venezuela. Our Maximum Likelihood phylogenetic analyses of cytochrome-b gene sequences recovered M. nesopolus as a paraphyletic group, with M. n. nesopolus and M. n. larensis as non-sister lineages. The haplotype network indicates that these two subspecies do not share any haplotypes and are in different evolutionary trajectories. Additionally, these two subspecies can be distinguished on the basis of qualitative and quantitative morphological traits. This pattern supports the recognition of M. nesopolus and M. larensis as full species. Our results also reveal that the assemblage of Caribbean Myotis do not form a monophyletic group. Caribbean species are phylogenetically close to mainland species from northern South America and Central America, suggesting that colonization of Caribbean islands happened multiple times.

Baird et al. (2015) split Lasiurus into three distinct genera (Aeorestes, Dasypterus and Lasiurus) based on tree topology and divergence times for the tribe Lasiurini. This arrangement has not been widely adopted by the scientific community and was criticized by Ziegler et al. (2016). More recently, Baird et al. (2017) reinforced the taxonomic arrangement of Lasiurini comprised by three genera. Baird et al. (2015, 2017) provided the most comprehensive phylogenetic analyses of Lasiurus and offer important insights on the phylogeny and alpha-taxonomy of the group. However, we disagree with the taxonomic arrangement proposed at the genus level and explain our point below.

The genus Myotis comprises a diverse group of vespertilionid bats with worldwide distribution. Twenty-eight Neotropical species are currently recognized. In this study, we evaluate molecular and morphological variation in the M. keaysi complex, a high elevation clade of Neotropical myotine bats characterized by complex taxonomy and high morphological variation. A phylogeny inferred with cytochrome-b sequences recovered two clades composed of samples traditionally assigned to M. keaysi, with 9% of genetic divergence between them. These clades were also suggested as putative distinct species by molecular species delimitation methods. Qualitative and quantitative morphological analyses indicated a phenotypic discontinuity between specimens from central Andes (including the holotype of M. keaysi) and western lowlands of Ecuador, showing strong congruence between molecular and morphological approaches. We therefore describe a new species for the Tumbes-Choco-Magdalena region, documenting their external and cranial diagnostic characters by comparing them with other Neotropical species. In addition, we provide an emended diagnosis for our new concept of M. keaysi.

Myotis lavali was described from the M. nigricans complex based on museum specimens from the Caatinga of northeastern Brazil. Current records of the species from about 10 localities suggest that M. lavali seems to occur throughout the South American dry corridor, with peripheral records in the Atlantic Forest. Based on new distribution records, we assess if M. lavali is endemic to the South American dry diagonal corridor through species distribution modeling using an ensemble approach. We obtained 35 occurrences for the species and built a consensus scenario based on 12 algorithms to model its distribution. Even using different thresholds to assign presence and absence of M. lavali, 95–99% of its predicted distribution is within the dry corridor, with few areas in the boundary of the Atlantic Forest, especially in deciduous forests. Therefore, our results support that M. lavali is indeed very likely restricted to the dry diagonal and, although it occupies forested areas, the core of its distribution lies in the Caatinga and in the Cerrado regions of Brazil.