The chemistry of copper and iron plays a critical role in normal brain function. A variety of enzymes and proteins containing positively charged Cu+, Cu2+, Fe2+, and Fe3+ control key processes, catalyzing oxidative metabolism and neurotransmitter and neuropeptide production. Here, we report the discovery of elemental (zero–oxidation state) metallic Cu0 accompanying ferromagnetic elemental Fe0 in the human brain. These nanoscale biometal deposits were identified within amyloid plaque cores isolated from Alzheimer’s disease subjects, using synchrotron x-ray spectromicroscopy. The surfaces of nanodeposits of metallic copper and iron are highly reactive, with distinctly different chemical and magnetic properties from their predominant oxide counterparts. The discovery of metals in their elemental form in the brain raises new questions regarding their generation and their role in neurochemistry, neurobiology, and the etiology of neurodegenerative disease.
Establishing the composition and distribution of chemical compounds within biological materials is fundamental to understanding the chemistry that drives life. Additionally, region-specific changes in chemical homeostasis have been linked to disease states [1]. Traditional techniques used to explore these relationships rely on the use of fixatives and dyes, which can significantly alter the native chemistry of the sample material [2]. Here we describe methodology for the preparation and examination of biological sample materials, including human tissues, using the X-ray Spectromicroscopy technique Scanning Transmission X-ray Microscopy (STXM). Through STXM we show the distribution and speciation of organic and inorganic materials within biological samples at a nanoscale resolution. Importantly this approach does not require the use of dyes, aldehyde fixatives or contrast agents and therefore offers an unprecedented insight into the native chemistry of biological samples. Human brain tissues were ethanol dehydrated and embedded in a resin comprised of equimolar trimethylolpropane triglycidyl ether: 4,4'-methylenebis (2-methylcyclohexylamine). Semi-thin (typically 200-500 nm) sections from embedded samples were cut using a non-metallic blade, before being mounted onto a support. STXM was performed at the carbon K-edge, oxygen K-edge, calcium L-edge and iron Ledge. To map distributions of chemical species, paired images were taken at the energy corresponding to a peak feature of interest and an off-peak energy a few eV away from this feature. The off-peak image is then subtracted from the peak image to give a difference map. This process is demonstrated in Figure 1, for a 200 nm thick section of human substantia nigra tissue imaged at the carbon K-edge. Here the peak image (288.3 eV; left) corresponds to the 1s to π* transition for amide groups (proteins), whereas the offpeak image (290 eV; middle) corresponds to the principal carbon K-edge absorption feature for the resin (see also [1]). This image processing allows artifacts and background resin absorption features to be removed, revealing the true tissue structure. From these difference maps, tissue structure is shown to be well preserved over the 160 µm 2 area presented. The high resolution map taken at ca. 40 nm resolution (right; inset) shows cellular structures, also displaying subcellular organelles.Further speciation maps and a carbon K-edge absorption spectrum from a 200 nm thick section of human amygdala tissue are shown in Figure 2. X-ray absorption spectra were obtained from a series of images taken at energies spanning the carbon K-edge edge. The resulting spectrum from tissue area A1 ( Figure 2f; green spectrum) was consistent with a synthetic peptide standard (Figure 2f; black spectrum), indicating the tissue to have been preserved through the embedding process. Further dense areas of tissue (as evidenced by enhanced contrast in Figure 2a) were found to be loaded with calcium (Figure 2b), carbonates ( Figure 2c) and iron (Figure 2d). This demonstra...
A hallmark of Parkinson's disease is the death of neuromelanin‐pigmented neurons, but the role of neuromelanin is unclear. The in situ characterization of neuromelanin remains dependent on detectable pigmentation, rather than direct quantification of neuromelanin. We show that direct, label‐free nanoscale visualization of neuromelanin and associated metal ions in human brain tissue can be achieved using synchrotron scanning transmission x‐ray microscopy (STXM), through a characteristic feature in the neuromelanin x‐ray absorption spectrum at 287.4 eV that is also present in iron‐free and iron‐laden synthetic neuromelanin. This is confirmed in consecutive brain sections by correlating STXM neuromelanin imaging with silver nitrate‐stained neuromelanin. Analysis suggests that the 1s–σ* (C−S) transition in benzothiazine groups accounts for this feature. This method illustrates the wider potential of STXM as a label‐free spectromicroscopy technique applicable to both organic and inorganic materials.
Biometals such as iron, copper, potassium, and zinc are essential regulatory elements of several biological processes. The homeostasis of biometals is often affected in age-related pathologies. Notably, impaired iron metabolism has been linked to several neurodegenerative disorders. Autophagy, an intracellular degradative process dependent on the lysosomes, is involved in the regulation of ferritin and iron levels. Impaired autophagy has been associated with normal pathological aging, and neurodegeneration. Non-mammalian model organisms such as
Drosophila
have proven to be appropriate for the investigation of age-related pathologies. Here, we show that ferritin is expressed in adult
Drosophila
brain and that iron and holoferritin accumulate with aging. At whole-brain level we found no direct relationship between the accumulation of holoferritin and a deficit in autophagy in aged
Drosophila
brain. However, synchrotron X-ray spectromicroscopy revealed an additional spectral feature in the iron-richest region of autophagy-deficient fly brains, consistent with iron–sulfur. This potentially arises from iron–sulfur clusters associated with altered mitochondrial iron homeostasis.
Lipopolysaccharide (LPS), an endotoxin, induces systemic inflammation by injection and is thought to be a causative agent of chronic inflammatory diseases, including type 2 diabetes mellitus (T2DM). However, our previous studies found that oral LPS administration does not exacerbate T2DM conditions in KK/Ay mice, which is the opposite of the response from LPS injection. Therefore, this study aims to confirm that oral LPS administration does not aggravate T2DM and to investigate the possible mechanisms. In this study, KK/Ay mice with T2DM were orally administered LPS (1 mg/kg BW/day) for 8 weeks, and blood glucose parameters before and after oral administration were compared. Abnormal glucose tolerance, insulin resistance progression, and progression of T2DM symptoms were suppressed by oral LPS administration. Furthermore, the expressions of factors involved in insulin signaling, such as insulin receptor, insulin receptor substrate 1, thymoma viral proto-oncogene, and glucose transporter type 4, were upregulated in the adipose tissues of KK/Ay mice, where this effect was observed. For the first time, oral LPS administration induces the expression of adiponectin in adipose tissues, which is involved in the increased expression of these molecules. Briefly, oral LPS administration may prevent T2DM by inducing an increase in the expressions of insulin signaling-related factors based on adiponectin production in adipose tissues.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.