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Background:Candida Associated Denture Stomatitis is the prevalent fungal pathosis in denture wearers, especially in immunocompromized patients. Existing antifungal agents are ineffective since the Candida species become resistant and also, they become toxic. Origanum vulgare is a herbal plant with high anti-fungal activity against Candida of blood and urine origin. However, it has never been explored against Candida from oral cavity.Materials & Methodology:Dry leaves of the plant were purchased and authenticated. Oil extraction was done using Hydro-distillation method. Clinical isolates of Candida from denture wearers was speciated using CHROMagar. Well Diffusion test was used to confirm the antifungal activity. Hydro-distillation & Maceration methods of extraction were compared. MIC/MFC was determined using CSLI guidelines. Infra-Red Spectroscopy was used to identify the active functional group.Results:O.vulgare showed 30±3mm of zone of inhibition as against 19mm for fluconazole. The suitable extraction method was Hydro-distillation. MIC & MFC were found to be 0.024% and 0.097% respectively which was much lesser than for fluconazole (0.25%). The active functional group had chemically similar structure as Carvacrol, usually found in antifungal herbs.Conclusion:within the limitations of the study, it was concluded that (a)O.vulgare is anticandidal for clinical isolates of oral Candida, (b) Hydro-distillation is an effective method as compared to Maceration (c) MIC & MFC are much lower than that of fluconazole (d) the major functional group was structurally similar to Carvacrol.

Abstract:Conventional complete denture therapy is the most extensively used form of treatment for replacement of missing dentition. Patient satisfaction plays a major role in determining the success during treatment procedures. It helps in assessment of certain psychological traits and the impact of complete denture prostheses on daily living. This study was done to evaluate the relationship between patient satisfaction and the newly constructed denture prostheses, as it would indicate the degree of success of the treatment given.

A denture wearer is highly prone to develop Candida associated denture stomatitis (CADS), due to the conversion of the normal oral commensal Candida spp. into a pathogen under favorable conditions. Immuno-compromised status, trauma from the prosthesis, other systemic conditions and improper maintenance of the dentures by the patient are few of the causative agents which turn the oral balance into an unhealthy and unsuitable foundation for the wearing of the prosthesis. Prevalence of denture stomatitis caused by the fungi Candida has been identified worldwide and has been reported to be around 65 to 70% of the denture wearers. The main species that causes infection has been Candida albicans, however, recently, there is a shift to non-Candida albicans-Candida (NCAC).This cross-sectional survey was undertaken to find the prevalence of denture stomatitis and the causative species in the south westcoastal region of Karnataka.

Shade selection procedure depends on various factors including translucency, contour and surface texture. Tooth shade selection using a conventional means involves a high degree of subjectivity. Traditional shade guides are available that use several methods for quantifying shade. Technology-based systems provide with an advantage of natural looking restorations. They include RGB devices, colorimeters, spectrophotometers. The impact of the color science can be seen on various restorative materials ranging from ceramics to maxillofacial prosthetic materials.

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Context: During natural calamities like fire accidents, many times the human body gets charred beyond recognition. Tooth, being the hardest structure in the human body protects the pulp within from such accidents. Forensic identification through the pulpal DNA could be avery useful tool in such situations. Aim: This study was designed to evaluate the quantity of DNA obtained from the pulp when exposed to varying temperatures. Methods and Material: Extracted teeth were subjected to the following temperatures:-80°C,37°C,100°C, 200°C, 300°C, 500°C and 1000°C. Pulp from these teeth were retrieved by horizontally sectioning the teeth using a fine needle diamond point in a high speed air rotor, followed by extraction of DNA with the HipuraA™ Forensic Genomic DNA purification spink it. The samples were quantified using personal computer(PC) based double beam spectro photo meter. Kruskal Wallis test and Mann WhitneyU test were used for statistical analysis. Results: Statistically significant difference (p<0.005) was seen in the quantity of DNA obtained from pulp subjected to higher temperatures as compared to oral temperature. However, there was no significant difference (p>0.05) in the quantity of DNA obtained from teeth subjected to-80°C Conclusions: Increase in temperature decreases the amount of DNA from the tooth pulp where as a decrease in temperature does not cause any change in the quantity of DNA.