Background Staphylococci are Gram-positive cocci arranged in clusters. They are colonized in humans and animals. Also, Staphylococcus aureus (S. aureus) is frequently associated with various superficial to deep-seated infections in humans. Due to the potential for easy transmission, Staphylococci are associated with both hospital-acquired and community-associated infections. Strains of S. aureus resistant to methicillin (MRSA) pose treatment challenges. In such cases, vancomycin is the treatment of choice. Due to the indiscriminate use of vancomycin, recently, we are seeing the emergence of vancomycin-intermediate sensitive S. aureus (VISA) and vancomycinresistant S. aureus (VRSA). The present study aims to evaluate the minimum inhibitory concentrations (MICs) of vancomycin and daptomycin among MRSA strains isolated from human clinical specimens Methods The study included 115 MRSA isolates collected over 26 months from July 2010 to September 2012. The strains were isolated from pus, urine, wound swabs, catheters, blood, and sputum. The bacteria were acquired from different inpatient and outpatient departments of Prathima Institute of Medical Sciences, Karimnagar, Telangana, India. Kirby-Bauer disk diffusion method using cefoxitin was used to confirm the MRSA isolates. The agar dilution and the Epsilometer method (E-test) were used to test the MICs of MRSA isolates against vancomycin and daptomycin, respectively, by the standard procedures recommended by the clinical laboratory standards institute (CLSI). Results Of the 115 S. aureus isolates, seven (6.08%) strains were resistant to vancomycin (VRSA) and 53 (46.08%) were found to be VISA using the new CLSI breakpoints. The MIC of the daptomycin was found to be ≤1 µg/ml for all the MRSA isolates. Conclusion The study results depicted an increasing trend in the vancomycin MICs among the MRSA isolates. Several tested strains show MICs in the intermediate sensitive range (VISA). The 1 2 3
Many parasitic infections essentially pose a significant public health problem throughout the world. However, the consequence of the issue is felt more among developing countries like India. This is majorly due to unsatisfactory hygienic practices and overcrowding, among others. Moreover, taeniasis caused by the parasites Taenia solium and Taenia saginata is a zoonotic infection transmitted via pigs and cattle, respectively. Even though the animals are the primary hosts where the parasite lives, humans also can suffer from infestation after consuming the eggs in contaminated food and water. Interestingly, there is a lower level of awareness regarding such types of Taenia infections in humans. On the contrary, most physicians and some people know that parasite transmission generally happens from the consumption of raw/undercooked pork and beef, which may result in a more serious consequence like neurocysticercosis. However, Taenia infestation in the intestines equally affects humans where people become reservoirs of infection, and suffer from severe morbidity. We present a case of worm infestation in a teenage girl who presented with severe peri-anal itching and motile worms in the stools.
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