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The nutritional composition of the edible seaweeds Codium fragile, Gracilaria chilensis and Macrocystis pyrifera was determined, including amino acid and fatty acid contents along with tocol and carotenoid contents. The results indicated that the three algae C. fragile, G. chilensis and M. pyrifera showed a high range of protein contents (13.7–10.8%), amino acid contents (1879.6–1417.7 mg/100 g dry algae) and a low content in lipids (0.7–15.0%). The most abundant fatty acids in C. fragile and G. chilensis were linolenic, oleic, and linoleic acid. d-Tocopherol and a-tocopherol (677.8 and 453.5 mg/g lipid, respectively) were found in C. fragile, while in G. chilensis and M. pyrifera, g-tocotrienol and a-tocopherol (263.5 and 1327.7 mg/g lipid, respectively) were found. In addition, in C. fragile and G. chilensis b-carotene was the principal carotenoid found (197.9 and 113.7 mg/g dry algae, respectively) compared with M. pyrifera (17.4 mg/g dry algae). The composition of macronutrients (minerals, carbohydrate-type dietetic fiber, proteins; low in lipids) and micronutrients (essential amino acids, PUFA of balanced n-6/n-3 proportion, b-carotene and a-tocopherol as source of vitamins A and E), corroborate the nutritional and biological potential of the studied algae, which constitute useful raw materials for the development of diets or ingredients for human and animal nutrition.This study was funded by Dirección de Investigación y Desarrollo DID, Universidad de Chile under Project DID SAL 02/14-2

Obesity is characterized by an increase in the infiltration of monocytes into the adipose tissue, causing an inflammatory condition associated with, for example, the development of insulin resistance. Thus, anti-inflammatory-based treatments could emerge as a novel and interesting approach. It has been reported that Chilean native fruits maqui (Aristotelia chilensis) and calafate (Berberis microphylla) present high contents of polyphenols, which are known for their antioxidant and anti-inflammatory properties. The aim of this study was to evaluate the ability of extracts of these fruits to block the pathogenic interaction between adipocytes and macrophages in vitro and to compare its effect with blueberry (Vaccinium corymbosum) extract treatment, which has been already described to possess several biomedical benefits. RAW264.7 macrophages were treated with 5 μg/mL lipopolysaccharides (LPS), with conditioned media (CM) from fully differentiated 3T3-L1 adipocytes, or in a coculture (CC) with 3T3-L1 adipocytes, in the presence or absence of 100 μM [total polyphenolic content] of each extract for 24 h. The gene expression and secretion profile of several inflammatory markers were evaluated. Nitric oxide secretion induced by LPS, CM, and CC was reduced by the presence of maqui (-12.2%, -45.6%, and -14.7%, respectively) and calafate (-27.6%, -43.9%, and -11.8%, respectively) extracts. Gene expression of inducible nitric oxide synthase and TNF-α was inhibited and of IL-10 was induced by maqui and calafate extract incubation. In conclusion, the extracts of these fruits present important inhibitory-like features over the inflammatory response of the interaction between adipocytes and macrophages, comprising a potential therapeutic tool against comorbidities associated with obesity development.

Rancidity development during the chilled storage of farmed Coho salmon (Oncorhynchus kisutch)Coho salmon (Oncorhynchus kisutch) is a fatty fish species whose farming production has greatly increased in recent years. Lipid damage produced during Coho salmon chilled storage was studied for up to 24 d. Lipid hydrolysis (free fatty acids, FFA) and oxidation (conjugated dienes; peroxide value, PV; thiobarbituric acid index, TBA-i; fluorescent compounds formation, FR; browning development) were determined and compared to lipid composition (polyene index, PI; astaxanthin, AX) changes and sensory assessment (rancid odour development) results. Most lipid damage indices developed slowly during storage; thus, values obtained for FFA, PV, TBA-i and FR were in all cases under 1.5 g/100 g, 4.0 meq oxygen/kg lipid, 0.40 mg malondialdehyde/kg muscle and 0.40, respectively. Odour assessment showed a significant (p ,0.05) rancidity development at day 10, when compared to starting fish material; then, nonacceptable values were obtained at days 19 and 24. The PI analysis showed not many differences during the storage time, with the lowest mean value at day 19. AX analysis indicated a relatively high content in the white muscle, which was maintained till the end of the experiment. A low oxidation development is concluded for Coho salmon lipids when compared to other fatty fish species under the same chilling conditions. AX was found to contribute to the oxidation stability of Coho salmon lipids, due to its free radical scavenger properties.

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Lipid changes related to quality loss were evaluated during frozen storage of coho salmon for up to 15 months. Biochemical indices concerning lipid hydrolysis (free fatty acids, FFA) and oxidation (peroxide value, PV; thiobarbituric acid index, TBA-i; fluorescent compounds, FR; polyene index, PI) were determined and compared to sensory (odor and taste) and endogenous antioxidant (tocopherol isomers and astaxanthin) assessments. As a result of the frozen storage, lipid hydrolysis was shown to develop according to the increase in FFA content (p < 0.05). However, most biochemical lipid oxidation indices (PV, TBA-i and FR) led to a low degree of rancidity development (p < 0.05) when compared to other fatty fish species under similar frozen storage conditions. The PI value decreased (p < 0.05) at month 10 but then remained unchanged until the end of the experiment. Rancid odor and taste development were shown to be low throughout the experiment, according to the biochemical indices mentioned above. However, a progressive decrease (p < 0.05) in the original fresh odor and taste of salmon fish flesh occurred with increasing frozen storage time, such that fish samples had the poorest scores by month 15. Endogenous antioxidants were remarkably stable throughout the experiment and which might contribute to the oxidative stability of frozen farmed coho salmon lipids.

Reductions on the sugars content of the food supply have been described after the initial implementation Chilean Labeling Law, but it is unclear if sugars were replaced by non-caloric sweeteners (NNS). We evaluated changes in the NNSs use in foods and beverages after the initial implementation of the labeling law. We used longitudinal data on packaged foods and beverages collected in six major supermarkets and three candy distributors in Santiago, Chile, in January–February 2015/2016 and in January–February 2017. We included in the analysis beverages, dairy-based beverages, yogurts, breakfast cereals, desserts and ice creams, candies and sweet confectioneries, and sweet spreads with a market share ≥1% of their food groups (n = 999). We compared the use of any NNS, the number of different NNSs used, and the combined use of NNSs and ingredients adding sugars using non-parametric tests for matched samples. We evaluated the association between a reduction in sugars and starting NNS use in the post-implementation period using negative binomial regression. The use of any NNS increased from 37.9 to 43.6% (p &lt; 0.001) after the law's implementation, NNSs increased among beverages, dairy-based beverages, yogurts, and desserts and ice creams (p &lt; 0.05), driven mostly by increases in sucralose and stevia use (p &lt; 0.05). We found that reformulated products that reduced the amount of sugars below the law's cutoff were more likely to start using an NNS in the post-implementation period (prevalence ratio: 12.1; 95%CI: 7.2–20.2; p &lt; 0.001). Our results suggest that NNSs likely replaced sugars after the initial implementation of the law. Further analyses should explore how these changes may impact NNS consumption.