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Degradation of both pseudouridine AF and As by periodate oxidation, borohydride reduct,ion, and alkaline hydrolysis gave 5-(1 ',2'-dihydroxyethy1)uracil. This establishes that the A isomers are anomeric forms of D-ribopyranosyluracil.When pseudouridine-C is heated in either acid or alkali three new isomers, designated AI., As, and B, are formed (Cohn, 1960;Chambers et al., 1963). The structure of pseudouridine-C has been unequivocally

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