The present study was undertaken over a three year period (2012–2014) in an organized dairy farm located in North India to ascertain Brucella abortus as the putative cause of abortion. The dairy farm maintained cattle of Frieswal, Crossbred and Sahiwal breeds and followed calf-hood vaccination with Brucella abortus Strain 19 live vaccine in all the heifers. Even with the recommended vaccination schedule and good managemental practices in place, 88 cases of abortions clinically suspected of bovine brucellosis (40 from Frieswal breed, 17 from Crossbred cattle and 31 from Sahiwal breed) were reported from this farm. From these abortion cases, bacteriological isolation was possible in only four dams while 16 dams were found to be serologically positive in Serum Tube Agglutination Test (STAT). Molecular screening by PCR assay (specific for the bcsp31 gene of B. abortus) revealed that 24 dams were positive, out of which 20 were from Frieswal breed and rest four were from Crossbred herd. Prominently, all Sahiwal dams were found to be negative in bacteriological isolation and also in PCR assay. These results thus indicate towards the possibility of breed predisposition to abortions due to B. abortus infection. Statistical analysis by Fischer exact test (p < 0.01) too substantiated that breed susceptibility exists among these PCR positive cases. This study is novel as breed variation in abortions due to B. abortus in cattle is being documented for the first time. Seven representative PCR amplicons generated during the study were also sequenced and submitted to NCBI GenBank. Moreover, this study also accentuates the importance of PCR screening especially in vaccinated herd and raises concerns on over-dependence of serological assays when intensive vaccination is practised without any concomitant DIVA strategy. Thus, besides assisting in planning pragmatic control strategies against bovine brucellosis these findings are also imperative from ‘One Health’ context, also.
The decision of myomectomy is not usually taken by OBG specialist for uterine fibroids during pregnancy because of its complications which may become hazardous at times. This is why it is generally delayed until after delivery. The current case was a large, asymptomatic subserous uterine myoma diagnosed during pregnancy by ultrasound and successfully managed by antepartum myomectomy retaining the fetus alive in utero at 13 -14 weeks gestation. At term, the patient had spontaneous vaginal delivery of 3 kg male child. This case demonstrates that myomectomy during pregnancy in special circumstances in selected cases to prevent forthcoming events adversely affecting mother and fetus can be considered.
A total of 18 Kankrej cattle of 1 st to 4 th parity were selected for the study and distributed into three different groups based on their pre calving BCS (before 15 days of expected date of calving) namely G1(2.50 -3.00), G2 (3.25 -3.75) and G3 (4.00 and above) with six animals in each group. The average fortnight fat % in G1 was decreased up to 60 th day, remained same up to 75 th days and thereafter decreased, in G2 group decreased significantly (P<0.05) up to 60 th day and again increased up to 90 th day, where as in G3 group increased significantly (P<0.05) up to 60 th day and then decreased up to 90 th day. The average lactose % was 4.40±0.09in G3 group which were highest followed by in G2 and G1 group 4.27±0.009 and 4.19±0.009, respectively. The average TS content of milk changes with advance in lactation in G1 and G2 group animals, and were found to be non significant throughout the study period. The animals of G2 BCS group had shorter postpartum estrus period, a fewer services per conception, higher first service conception rate and a shorter service period followed by Kankrej cattle of G3 group and GI group. The studied revealed that BCS had a significant (P<0.05) effect on the reproductive performance in experimental animals.
The present investigation was carried out with the total of 18 Sahiwal cattle of 1 st to 4 th parity were selected for the study and distributed into three different groups based on their pre calving BCS (before 15 days of expected date of calving) namely G1 (2.50-3.00), G2 (3.25-3.75) and G3 (4.00 and above) with six animals in each group. The average fortnight milk yield increased up to 60 th day and thereafter declined up to 90 th day in G1, G2 and G3 group of animals, which was significant (p<0.05) up to 75 th day. G3 group animals had highest average fortnightly cumulative Milk yield (Kg) in entire study period. Highest 305 days predicted lactation milk yield (2462.66 Kg /lactation) and average peak yield (16.50 Kg/ animal) was obtained in G3 group animals and the lowest predicted lactation milk yield (1870.68 Kg/lactation). The 305 days predicted lactation milk yield in G2 group animals was 2356.75 Kg /lactation and average peak yield was 14.90 Kg/ animal. The G3 group animals also attained their peak yield in 46 days compared to G2 and G1 group animals with 51 and 54 days, respectively. The persistency of milk production was highest in G2 group animals with 65.63 % followed by G3 and G1 group animals. The studied revealed that BCS had a significant (P<0.05) effect on post-partum estrus and service period in experimental animals.
Cesarean section in buffaloes is an emergency operative procedure being performed principally for uncorrectable uterine torsions and for delivery of fetal monsters. Ten cases of uncorrectable uterine torsion and fetal abnormalities were reported at TVCC, IIVER, Rohtak. It was decided to perform cesarean section in all the cases. Buffaloes were restrained in left lateral recumbency with both forelimbs and hindlimbs tied separately. Cesarean sections were performed under local infiltration analgesia using 2% lignocaine HCl and sedation with triflupromazine. Uterus were sutured using absorbable suture material with chromic catgut no. 2 in all the cases. Before suturing, available antibiotic pessaries were placed in the uterus. Post-operatively fluid therapy (5 days), broad spectrum antibiotics (5 days), NSAIDS (3 days) along with multivitamin (3 days) were administered. All animals except one recovered uneventfully.
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