Pituitary extracts from 560 mature Ku.n.u h.exadactylu of both sexes from winter and summer seasons were collected locally and were injccted into frogs from summer and winter months. The adenohypophysial gonadotrophes were studied from normal animals and from thc animals injected wjth pituitary extracts. The pituitary cell types changed in experimental frogs in comparison to the corresponding cells of normal animals. An attempt has been made to correlate the changes of gonadotrophes with changes in spermatogcnetic proliferation as reported earlicr (Kasinathaii and Basu, '72).
Prostate glands from mature and juvenile Cryptozollo belanqcri were cultured ill ritro for 8 days in single or double association with cerebral ganglia. visceral ganglionic complex and gonads obtained from adult snails. The histology of the glands was studied and compared with those of normal glands and with glands cultured ill ritro without any organ association. Functional differentiation was not evident in glands cultured in isolation or in single association with gonad or brain. In double associations, involving cerebral ganglia, visceral ganglionic complex and gonad response from the gland was obtained only when the gonad was involved. The cerebral ganglia and visceral ganglionic complex acting through the gonad elicit differential responses from the prostate gland. It was concluded from the in ritro studies that the prostate gland may be dependent on a gonadal hormone for functional differentiation. which in turn is under the control of trophic factors released from the brain.
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