Background:Fixation is the most imperative step in the practice of diagnostic histopathology, which is intimately linked to 10% formalin. As a result of increasing concerns about the potential carcinogenicity of the formaldehyde, attempt to find safer alternatives is necessary. Honey has been shown to possess antimicrobial, antiviral and antimutagenic properties. Many studies have reported that honey possesses dehydrating and preserving effects also.Aims and Objectives:To study the fixative properties of processed and unprocessed honey in oral tissues followed by comparision with formalin.Materials and Methods:The study group comprised 12 different tissues. Each tissue was cut into 3 segments and were immediately fixed in bottles containing 10% unprocessed honey, 10% processed honey and 10% formalin, respectively, for 24 h at room temperature. After fixation, tissues were processed using the routine standard processing protocol followed by hematoxylin and eosin staining. Data were statistically analyzed using descriptive statistics such as mean, standard deviation and percentage. Kruskal–Wallis test followed by post hoc Dunn's multiple comparisons test and Chi-square test or Fisher's exact test for small sample size. A P < 0.05 was considered as significant. Data analysis was done by using software Minitab v14.0.Results:When all the stained sections were assessed for the parameters, there was no statistically significant difference between tissues fixed in processed and unprocessed honey compared to formalin (P = 0.004). The tissue morphology and staining adequacy for diagnosis in honey fixed tissue was at par with formalin fixed tissue. Hence, our results suggest that both processed honey and unprocessed honey can be used as a safe alternative for formalin.
Background: Researchers today are driven toward the medicinal and therapeutic value of the natural products like Aloe vera, basil, turmeric, green tea, pomegranate (Punica granatum) to prevent dental caries. The aim of the present study was to determine the antimicrobial activity of pomegranate aril extract on streptococci in dental caries patients and healthy individuals. Materials and Methods:The study group included 40 subjects; age comprised of 18-26 years 20 dental caries patients were taken as an experimental group (after observing the decayed missing fi lled teeth index) and 20 healthy individuals without caries as a control group. Two samples were collected from each subject. The collected sample was streaked on Mitis Salivarius Agar, which was incubated at 37°C for 24 h. The colony forming units (CFU) were counted using manual colony counter, and the data were analyzed using paired t-test and unpaired t-test. Results:The results obtained after pomegranate swish in the experimental group showed a percentage reduction of 52.2% (P < 0.0001) and 33.8% (P < 0.0001) in control group for the CFU. The comparison of after sample between the experimental group and the control group was highly signifi cant. Conclusion:The result obtained in the study clearly demonstrates that the pomegranate aril extract has an antimicrobial eff ect against Streptococcus, thus acting as an anti-cariogenic agent.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.