The ozonation process has been widely used as an alternative in hospital waste water treatment because of ozone as a strong oxidizing agent which is effective in degrading unsaturated pollutant bonds, as well as breaking down complex organics. Combination of Ozone, CaO, and Moringa seed (Moringa oleifera) as alternative technology has functions to reduce BOD and COD in hospital wastewater. This study was conducted to determine the effectiveness application of ozone technology combined with CaO and Moringa seed. The ozonation contact time was 45 minutes, CaO concentration 4% at 3 ml/l, 2 g/l in Moringa seed, and combination of CaO and Moringa seed (v/w) of 1.5:1. This type of research is a quantitative research based pure experimental method with pretest-posttest design on a laboratory scale. Samples used by hospital Bung Karno, Surakarta, Central Java. The results showed at ozone levels of 0.058 - 0.064 g/hour combined with CaO and Moringa seed (v/w) 1.5:1 was able to produce an effective reduction BOD levels by 56.00% and the effectiveness of reducing COD levels to 63.57%. Univariate analysis showed that there was an effect on the COD and BOD parameters were significantly different with p-value < 0.05 using ozonation treatment combined with CaO:Moringa seed (v/w) 1.5:1. The mean result of COD level after treatment was 156.47 + 2.11 mg/l and BOD level after treatment was 47.42 + 1.81 mg/l.
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