A 7-year-old female neutered Jack Russell Terrier was presented to Langford Vets, the University of Bristol, with a history of chronic intermittent lethargy. Investigations and clinical course were compatible with hereditary hemolysis due to a red blood cell membrane defect. Proteomics was used to explore protein alterations in the presence of a hypothesized red blood cell membrane protein deficiency. Proteomic analysis revealed downregulation of the band 3, and alpha- and beta-adducin proteins, and alterations in the red blood cell proteome consistent with previous reports of changes due to the presence of reticulocytosis and ongoing hemolysis. The spectrum of protein alterations identified in the affected dog may be homologous to a band 3 protein deficiency secondary to hereditary spherocytosis, as described in people.

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transfusion reaction. 25/30 adverse events occurred after a pRBCs transfusion; 5/30 after WB transfusion. The portion of adverse reactions was (37/129) with pRBCs and (7/36) after WB. 23/44 (52%) reactions occurred in dogs receiving single transfusions; 17/44 (38%) occurred after multiple transfusions. Of the 43 dogs receiving multiple transfusions, 10 (23%) had repeated reactions. STATEMENT (CONCLUSIONS)Immune-mediated disease was the commonest cause of transfusion-dependent anaemia. Treatment with corticosteroids did not decrease the incidence of transfusion reactions. Performance of more than one transfusion was not associated with an increased risk of adverse reactions. OBJECTIVESTo evaluate whether prior corticosteroid administration influences imaging findings in dogs with immunemediated haemolytic anaemia (IMHA). METHODSRetrospective review of case records of a referral population of dogs with IMHA, for whom medical histories and thoracic and/or abdominal imaging studies were available.Cases were classified based upon presence (S+) or absence (S-) of corticosteroid administration prior to referral. Thoracic and abdominal imaging findings were categorized into (a) clinically significant findings altering case management or follow-up, including potential triggers; (b) incidental, clinically insignificant; (c) attributable to IMHA; (d) normal. For statistical analysis, significant imaging findings (a) versus non-significant imaging findings (b, c and d) were compared in S+ and S-dogs, using χ 2 test to determine whether prior corticosteroids significantly influenced imaging findings. RESULTS55 dogs met the inclusion criteria, comprising 23 S+ and 32 S-. 46 had thoracic and abdominal imaging. 2 and 7 dogs had only thoracic or abdominal imaging, respectively. The 48 thoracic studies comprised 25 computedtomographic and 23 plain radiographic. The 53 abdom inal studies comprised 24 computed-tomographic and 29 ultrasonographic.

IP: 44.224.250.200 [ 460 ] BSAVA CONGRESS 2018 PROCEEDINGS confirmed on blood film evaluation. Cats with a normal platelet count were selected for comparison. Cats were categorized for data analysis based on final diagnosis and mechanism of thrombocytopenia.