. In all cases, ⅐ OH radicals were linearly produced, with the highest rate obtained with MD, followed by DBQ, MBQ, and BQ. These rates correlated with both H 2 O 2 levels and Fe 3؉ reduction rates observed with the four quinones. Between the two P. eryngii mycelia used, the best results were obtained with the one producing only laccase, showing higher ⅐ OH production rates with added purified enzyme. The strategy was then validated in Bjerkandera adusta, Phanerochaete chrysosporium, Phlebia radiata, Pycnoporus cinnabarinus, and Trametes versicolor, also showing good correlation between ⅐ OH production rates and the kinds and levels of the ligninolytic enzymes expressed by these fungi. We propose this strategy as a useful tool to study the effects of ⅐ OH radicals on lignin and organopollutant degradation, as well as to improve the bioremediation potential of white-rot fungi.
Oxygen activation during oxidation of the lignin-derived hydroquinones 2-methoxy-1,4-benzohydroquinone (MBQH 2 ) and 2,6-dimethoxy-1,4-benzohydroquinone (DBQH 2 ) by laccase from Pleurotus eryngii was examined. Laccase oxidized DBQH 2 more efficiently than it oxidized MBQH 2 ; both the affinity and maximal velocity of oxidation were higher for DBQH 2 than for MBQH 2 . Autoxidation of the semiquinones produced by laccase led to the activation of oxygen, producing superoxide anion radicals (
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