HighlightThe computed tomography (CT) scanning method is used for the temporal analysis of panicle development in two rice varieties varying in their yield, in order to understand seed development and its correlation with growth parameters.

Abstract:Terminalia arjuna is one of the many such plant that used in indigenous system of medicine for curing different diseases. For effective formulation of herbal drug selection of proper age of the plant is imperative. In the present study, the T. arjuna plant (leaves) was collected from the Botanical garden of Saurashtra University. The leaves were grouped in to various size and growth analysis was performed. Each extract homogenized in methanol and was used for chlorophyll estimation and antioxidant activities. Radical scavenging activity of the methanol extract of different stages leaves were determined using a stable free radical 2, 2-diphenyl-1-picryl hydrazyl (DPPH), using Micro plate assay. Further the phenolic compounds were separated by Reverse phase high pressure liquid chromatography analysis (RP-HPLC). At the leaf stage when maximum antioxidant activity was observed, phenol like Gallic acid showed distinct peak. The role of phenolic compounds in antioxidant activities is discussed.

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Indole-3-acetic acid (IAA) and phenyl acetic acid (PAA) are two major physiologically active auxins, found in higher plants. The objective of this study was to evaluate changes in the endogenous levels of IAA and PAA in rice (Oryza sativa) seed and predict probable transporter and efflux (signaling) proteins for docking with auxins from the available rice genome data. IAA and PAA were estimated during rice seed development using sensitive and specific antibodies against each hormone. The levels of IAA and PAA were higher when sink size development was at its peak, suggesting an important role played by the auxin in sink size development of rice seed. Furthermore, auxin binding transporters proteins of rice were confirmed for docking purpose and compared with Arabidopsis and maize proteins. In this context, PAA docked with known IAA proteins; although, docking score of IAA was higher than that of PAA in rice seed. Twelve genes of the auxin efflux carrier (PIN family) of rice were also analysed for phylogeny relatedness with Arabidopsis and maize, to further understanding auxin concentrations and efflux within rice seeds. The predicted PIN proteins for IAA efflux of rice showed docking affinities with PAA, revealed its role in maintaining physiological concentrations of auxins in rice seeds.