We present an optimized scheme for nanoscale measurements of temperature in a complex environment using the nitrogen-vacancy center in nanodiamonds (NDs). To this end we combine a Ramsey measurement for temperature determination with advanced optimal control theory. We test our new design on single nitrogen-vacancy centers in bulk diamond and fixed NDs, achieving better readout signal than with common soft or hard microwave control pulses. We demonstrate temperature readout using rotating NDs in an agarose matrix. Our method opens the way to measure temperature fluctuations in complex biological environment. The used principle is universal and not restricted to temperature sensing.
Mechanobiology seeks to understand how cells integrate their biomechanics into their function and behavior. Unravelling the mechanisms underlying these mechanobiological processes is particularly important for immune cells in the context of the dynamic and complex tissue microenvironment. However, it remains largely unknown how cellular mechanical force generation and mechanical properties are regulated and integrated by immune cells, primarily due to a profound lack of technologies with sufficient sensitivity to quantify immune cell mechanics. In this review, we discuss the biological significance of mechanics for immune cells across length and time scales, and highlight several experimental methodologies for quantifying the mechanics of immune cells. Finally, we discuss the importance of quantifying the appropriate mechanical readout to accelerate insights into the mechanobiology of the immune response.
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