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The ability of three milk-based extenders to preserve the motility and viability of cooled shipped semen produced by stallions after a long sexual rest was evaluated. In total, 21 ejaculates from 11 stallions were collected after 5-6 months of sexual inactivity and diluted with Kenney, INRA 96, and EquiPlus. Seven parameters were evaluated -spermatozoa viability (VIT, %), total motility (MOT, %), progressive motility (PMOT, %), number of progressive spermatozoa per ml (prog M/ml, million/ml), average path velocity (VAP, μm/s), curvilinear velocity (VCL, μm/s) and straight-line velocity of spermatozoa (VSP, μm/s). These parameters were determined by CASA 2 hours after semen processing and then in 24, 48 and 72-hour intervals of storage at 5 °C. The effect of storage time was highly significant (P<0,001) for all evaluated parameters. The effect of the extender was significant (P<0.05) for VIT, MOT, PMOT, VCL, VSL and VAP. The ejaculates extended with INRA 96 reached significantly (P<0.01) higher values for all evaluated parameters compared to Kenney extender. The INRA 96 extender significantly increased the values of PMOT, VCL and VAP compared to EquiPlus (P<0.05). In all observed extenders, MOT and VIT decreased linearly whereas PMOT decreased exponentially.The fastest decrease of PMOT was observed within 24 hours of storage. The results obtained from using INRA 96 showed that quality of cooled semen for stallions collected after a long period of sexual inactivity can be sufficiently maintained during at least 24 hours of storage.

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