Nanodiamonds represent an attractive potential carrier for anticancer drugs. The main advantages of nanodiamond particles with respect to medical applications are their high compatibility with non-cancerous cells, feasible surface decoration with therapeutic and cancer-cell targeting molecules, and their relatively low manufacturing cost. Additionally, nanodiamond carriers significantly increase treatment efficacy of the loaded drug, so anticancer drugs execute more effectively at a lower dose. Subsequently, lower drug dose results in less extensive side effects. The carriers decorated with a targeting molecule accumulate primarily in the tumor tissue, and those nanodiamond particles impair efflux of the drug from cancer cells. Therapeutic approaches considering nanodiamond carriers were already tested in vitro , as well as in vivo. Now, researchers focus particularly on the possible side effects of nanodiamond carriers applied systemically in vivo. The behavior of nanodiamond carriers depends heavily on their surface coatings, so each therapeutic complex must be evaluated separately. Generally, it seems that site-specific application of nanodiamond carriers is a rather safe therapeutic approach, but intravenous application needs further study. The benefits of nanodiamond carriers are remarkable and represent a potent approach to overcome the drug resistance of many cancers.
Boron doped diamonds (BDD) are a promising material for fabrication of neuroelectrodes and formation of neurointerfaces. Surface properties of BDD thin films can be adjusted by variation of grain sizes, patterning, lithography techniques and coating by different biopolymers to further promote performance of neuroelectrode and enhance the neuron adhesion.We used 3T3 fibroblasts and rat hippocampal neurons (newborn and adult) to estimate planar and nanostructured BDD surface biocompatibility. Optical microscopy, lactate dehydrogenase assay and calcium imaging were used to check cell adhesion and longterm survival.3T3 fibroblasts are able to grow on BDD without any coating. No signs of cytotoxicity and changes in cell cycle were found. Adhesion and longterm survival of newborn rat hippocampal neurons were observed only on poly‐L‐lysine coated planar and nanostructured BDD surface. Calcium imaging of longterm cultures of newborn neurons grown on poly‐L‐lysine coated planar and nanostructured BDD showed functionality and synaptic activity of hippocampal neurons. Nanodiamond surface without poly‐L‐lysine coating do not support adhesion of newborn neurons.Adult rat hippocampal neurons preferred poly‐D‐lysine coated planar nanodiamonds but were able to grow on nanostructured diamonds without any coating.Support or Funding InformationThis work was supported by Czech Science Foundation grant 17–15319S.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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