RESUMO Visando a minimizar o período de tratamento cicatricial em diversos tecidos, buscam-se alternativas à terapia

Pesq. Vet. Bras. 32(10):980-986, outubro 2012 980 RESUMO.-[Transferência de imunidade passiva e proteinograma sérico de bezerros das raças Crioula lageana e Holandesa preta e branca nos primeiros seis meses de vida.] O objetivo do presente trabalho foi veriϐicar e comparar a transferência de imunidade passiva e o proteinograma em bezerros da raça Crioula Lageana (CL) e Holandesa Preta e Branca (HPB). Foram utilizados dois grupos sendo 13 bezerros da raça Crioula Lageana e 10 da raça HPB. Amostras de sangue foram colhidas para a mensuração da proteína sérica total, eletroforese das proteínas séri-cas, atividade da gama-glutamiltransferase e concentração de IgG pelo método de turvação pelo sulfato de zinco nos períodos entre 24 e 36 horas de vida, 15, 30, 60, 90, 120, 150 calves. Two groups were utilized with 13 Criollo Lageano and 10 BWH calves. Blood samples were collected for the measurement of total serum protein, electrophoresis of serum proteins, activity of the gamma glutamyl transferase, and concentration of IgG by the method of the zinc sulfate turbidity in periods between 24 and 36 hours of life, 15, 30, 60, 90, 120, 150 and 180 days. Statistical analysis was performed by ANOVA and Tukey test at 5% signiϐicance level, and correlations between variables were calculated. Variations of serum proteins followed a pattern of physiological behavior over the ϐirst six months of life and production of immunoglobulins was active earlier in BWH calves and slower in the Criollo Lageano, without causing any impact on their health. Gamma globulin in the ϐirst days of life (24-36h) was correlated with IgG (r=0.87 for CL and r=0.89 for BWH), PTS (r=0.91 for CL and r=0.92 for BWH), Glob (r=0.99 for CL and r=0.98 for BWH) and GGT (r=0.14 for CL and r=0.83 for BWH). It was concluded that there was no failure in the transfer of passive immunity in Criollo Lageano calves but this failure occurred in the BWH calves. IgG values estimated by the zinc sulfate turbidity and serum proteins were considered good indicators of the transfer of passive immunity in calves between 24 and 36 hours of life. de ANOVA e Tukey ao nível de 5% de signiϐicância, e correlações entre as variáveis. As variações das proteínas séricas obedeceram a um padrão de comportamento ϐisiológico ao longo dos seis primeiros meses de vida e a produção ativa de imunoglobulinas foi mais precoce nos bezerros da raça Holandesa e mais lenta nos da raça Crioula Lageana, sem provocar qualquer impacto sobre a saúde dos mesmos. A gamaglobulina no primeiro dia de vida (24-36h) correlacionou-se com a IgG (r=0,87 CL e r=0,89 HPB), PTS (r=0,91 CL e r=0,92 HPB), Glob (r=0,99 CL e r=0,98) e GGT (r=0,14 CL e r=0,83 HPB). Conclui-se que não houve falha na transferência de imunidade passiva nos bezerros da raça Crioula Lageana e falha na raça Holandesa Preta e Branca. Os valores de IgG estimados pelo método da turvação pelo sulfato de zinco e das proteínas séricas foram bons indicadores da transferência de imunidade passiva nos bezerros entre 24 e 36 horas de vida.TERMOS ...

RESUMO Para avaliar a cicatrização de feridas cirúrgicas da parede abdominal de ratos submetidos à celiotomia, utilizou-se a terapia com ultra-som (US) nos modos contínuo e pulsado. Foram utilizadas 45 ratas, separadas em três grupos INTRODUÇÃOOs procedimentos cirúrgicos realizados no interior da cavidade celômica e nas áreas retroperitoniais têm como via de acesso a parede abdominal, considerando-se a celiotomia uma das práticas cirúrgicas mais freqüentes (FRY & OSLER, 1991).Em humanos, a deiscência, caracterizada pela separação dos planos profundos, ocorre entre zero e 6% das celiotomias, com taxas de mortalidade que

Morphological changes in the placenta at periparturition and the involvement of apoptosis controlling cell populations in the placentome were evaluated in Holstein and Nelore cows. Twenty-two Holstein cows at the end of gestation with normal non-induced parturition (group I) and 10 Holstein cows with induced parturition (group II) were used in this study. In addition, groups III and IV were constituted by 10 Nelore cows at the end of gestation with normal non-induced parturition and 21 Nelore cows with induced parturition, respectivelly. Binucleate trophoblastic cells, epithelial caruncular cells and apoptotic bodies were quantified by light microscopy. Apoptosis was further evaluated by transmission electron microscopy, ELISA and DNA electrophoresis in agarose gel. The number of caruncular epithelial cells did not vary between breeds with normal or induced parturition. The number of binucleate cells was significantly different between Holstein and Nelore cows with normal non-induced parturition (groups I vs. III), but their numbers were not significantly different between breeds when parturition was induced. In cows with normal non-induced parturition, the intensity of apoptosis, as assessed by morphometric analysis and ELISA, was significantly higher in group I when compared to group III. However, no difference in the intensity of apoptosis was found between groups II and IV. Characteristic apoptotic ladder pattern of DNA fragmentation was detected by agarose gel electrophoresis. Ultra-structural features of apoptosis in maternal and fetal epithelium as well as capillary vascular endothelium were identified by transmission electron microscopy. In conclusion, in Nelore placental maturation occurs precociously when compared to Holstein. In both breeds, apoptosis is an active physiological event that seems to be required for maturation and normal post-partum release of the placenta.Keywords: bovine, placentome, binucleate cell, epithelial cell, apoptosis RESUMO Foram avaliadas as diferenças morfológicas e a participação da apoptose na população de células do placentoma de vacas das raças Holandesa e Nelore no período periparto. Foram utilizadas 22 vacas da raça Holandesa com parto a termo (tratamento I) e 10 com parto induzido (tratamento II). Nos tratamentos III e IV foram utilizadas 10 vacas Nelore com parto a termo e 21 com parto induzido, respectivamente. As células binucleadas trofoblásticas e epiteliais carunculares e os corpos apoptóticos

This research was performed by a multidisciplinary and interagency team and sought to determine the blood profile, total plasma protein and fibrinogen concentrations of clinically healthy adult Campeiro horses. A total of 138 horses (14 stallions, 74 non-pregnant and 50 pregnant mares)

This study aimed to describe and differentiate Crioulo Lageano and Nellore beef using chemical and sensory characteristics. Twenty-four cattle were divided into two groups as follows: Nellore (12) and Crioulo Lageano (12). Six types of cuts were analysed: topside, rum cap, eye round, tenderloin, sirloin and rib eye. The Crioulo cuts had a higher (P £ 0.05) percentage of fat compared with the Nellore cuts with the exception of the sirloin and rib eye where no significant differences were found. Moreover, breed type influenced collagen variation. The total colour difference parameter indicated a significant colour difference between meats from the different breeds. A quantitative descriptive analysis showed differences in sensory attributes between breeds and cuts. The breed type had a significant effect on the fibre residue, bitter taste, chewiness and fat residue. According to the principal component analysis, certain sensory attributes provided 48.8% of the information about the Crioulo meat.Mean values with different letters are significantly different (P £ 0.05) between breeds for each cut (n = 12).Chemical and sensory characteristics of meat M. L. Mitterer-Daltoé et al.Chemical and sensory characteristics of meat M. L. Mitterer-Daltoé et al.

SUMMARYThe effects of gestation and lactation on the dynamics of the thyroid hormones, triiodothyronine (T 3 ) and thyroxine (T 4 ) were evaluated in Crioula Lanada Serrana ewes from the plateau region in Santa Catarina -Brazil. To evaluate the effects of gestation, twenty four ewes were randomly divided into groups I and II. Group I was composed of 12 pregnant ewes and the control group (II) was composed of 12 non-pregnant ewes. The effects of lactation were evaluated in the same ewes of group I after parturition (LE) and in the control group (NL). Blood samples were taken in the first, second and third periods of gestation, at parturition, and at 30, 60 and 90 days of lactation. T 3 and T 4 concentrations were determined by radioimmunoassay (RIA). Pregnant ewes showed lower serum concentrations of T 3 and T 4 than those of non-pregnant ewes. At parturition, there was no significant difference in T 3 concentrations between groups I and II (p>0.05). During lactation, serum T 3 concentrations were lower (p≤0.05) in LE than in NL ewes. Serum T 3 concentrations were greater (p≤0.05) at lambing than during the lactation periods. Serum T 4 concentrations in LE at parturition did not differ (p>0.05) from those of NL ewes. During lactation, T 4 concentrations of LE were lower (p≤0.05) than those of the NL ewes. The results of this study have shown that gestation causes a variation in the T 3 and T 4 serum concentrations, and that lactation results in a decrease of serum concentrations of T 3 and T 4 in Crioula Lanada Serrana ewes. RESUMOOs efeitos da gestação e lactação sobre a dinâmica dos hormônios tireoidianos triiodotironina (T 3 ) e tiroxina (T 4 ) foram avaliados em ovelhas Crioula Lanada Serrana na região do Planalto Serrano Catarinense. Para avaliar os efeitos da gestação, foram utilizadas vinte e quatro ovelhas distribuídas aleatoriamente em dois grupos. O grupo I foi formado por 12 ovelhas gestantes e o grupo controle por 12 ovelhas não gestantes (grupo II). Os efeitos da lactação foram avaliados nas mesmas ovelhas do grupo I após a parição (LE) e no grupo controle (NL). Foram colhidas amostras sanguíneas no primeiro, segundo e terceiro terço da gestação, ao parto e aos 30, 60 e 90 dias de lactação. As concentrações de T 3 e T 4 foram determinadas por radioimunoensaio (RIA). As ovelhas gestantes apresentaram concentrações séricas de T 3 e T 4 inferiores àquelas não gestantes. Ao parto não houve diferença significativa nas concentrações de T 3 entre os grupos I e II (p>0,05). Durante a lactação as concentrações Archivos de zootecnia vol. 59, núm. 228, p. 510

The objective was to select and train assessors to evaluate metallic sensations in beef. The detection threshold of FeSO 4 .7H 2 O was determined using the method of limits. Twenty-six undergraduates participated in the test, and a single detection threshold was determined. Candidates with a threshold lower than or equal to the general value threshold were accepted. Next, the performance of the accepted candidates was evaluated using meat from the semimembranosus muscle of bovines at an internal cooking temperature of 65, 72 or 75℃. The metallic sensation in the meat was evaluated using the ranking test and an unstructured 9.0 cm line scale. The extremes of the scale have the expression not perceptible and highly perceptible. The assessors were selected to help the team analyze the discriminative power, reproducibility and overall use of scale. The results demonstrated that the processes for selecting and training assessors to evaluate the metallic sensation in meat were adequate.