The antibacterial activity and total phenolic (TP) content of Agaricus bisporus stipes were assessed using solvent and water extracts to determine its bioactivity. Extraction methods included accelerated solvent extraction (ASE) and hot water followed by membrane concentration. Water extract from ASE had the highest TP of 1.08 gallic acid equivalents (GAE)/g dry weight (DW) followed by ethanol at 0.61 mg GAE/g DW and 0.11 mg GAE/g DW for acetone. Acetone extracts inhibited Escherichia coli and Staphylococcus aureus at less than 50%; ethanol inhibited E. coli at 61.9% and S. aureus at 56.6%; and ASE water inhibited E. coli at 78.6% and S. aureus at 65.4%. The TP content of membrane concentrated extract of mushroom was 17 mg GAE in 100 mL. Membrane concentrated water extracts had a higher percentage inhibition on S. aureus than E. coli. Overall, the results were promising for further application of mushroom stipe extracts as a functional food additive.
PRACTICAL APPLICATIONSMushrooms are known for their health benefits and have been identified as a good source of nutrients. The highly perishable nature of mushrooms warrants further processing and preservation to minimize losses along the supply chain. This study explores the possibility of adding value to mushroom stipes, a by-product of the fresh mushroom industry. The extracts assessed indicate the antibacterial activity and phenolic content, and the potential of using these extracts as functional ingredients in the food industry. This study provides valuable information to the scientific community and to the industries developing novel ingredients to meet the market demand for natural food additives.
This study explores Rwandan ethno-veterinary knowledge and the degree to which this knowledge is reflected in the country’s technical and vocational education and training (TVET) instruction. The knowledge considered is the Indigenous medicinal knowledge used by rural Rwandan livestock farmers to treat their cattle. Through interviews with farmers, TVET graduates and TVET teachers, and an examination of the current TVET Animal Health curriculum, the research identifies a neglect of Indigenous knowledge in the curriculum, despite the fact that local farmers use numerous Indigenous medicinal innovations to treat their animals. The focus of the Rwanda’s TVET Animal Health curriculum is on Western-origin modern veterinary practices. The authors argue that this leaves Rwandan TVET Animal Health graduates unprepared for optimal engagement with rural farmers and with the full range of potential treatments.
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