down, the interaction of MBP-5HT 3A -ICD constructs and RIC-3 is investigated. Furthermore, we co-expressed 5-HT 3A and RIC-3 in Xenopus oocytes to study the interaction in-vivo by two electrode voltage clamp (TEVC) recordings. Full-length 5-HT 3A -mediated currents are significantly reduced when RIC-3 is co-expressed. Here, we study if individual MBP-5-HT 3A -ICD constructs rescue 5-HT 3A -mediated currents in the presence of RIC-3. Our results support the hypothesis that interaction of 5-HT 3A -ICD and RIC-3 is mediated by specific segments of the 5-HT 3A -ICD as opposed to the complete domain. Further studies are directed toward deciphering the molecular determinants of RIC-3 protein-protein interaction.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.