Salinity is one of the principal abiotic stresses that affect plant productivity by inducing osmotic stress, which in turn, causes oxidative stress. Plants respond to this oxidative stress by adjusting levels of antioxidants and associated components. 10-day old seedlings of Niger were evaluated for abiotic stress response in terms of antioxidants and antioxidant enzymes over 72 h in presence of up to 500 mM NaCl in combination with CaCl 2 . Stress markers: H 2 O 2 , lipid peroxidation, antioxidants; ASC and GSH and antioxidant enzymes such as POX, APX and GR were significantly elevated, while CAT was reduced. The response was concentration and time-dependent up to 300 mM NaCl and fluctuated beyond. Metabolic enzymes β-amylase and acid phosphatase exhibited moderate increase relative to controls. The parameters indicated tolerance of the plants to salinity up to 300 mM over 48 h.
MicroRNAs (miRNAs) are group of small, non-coding RNAs that play important roles in plant growth, development and stress response. There have been an increasing number of investigations aimed at discovering miRNAs and analyzing their functions in model plants. In this study, we constructed high temperature stress induced small RNA libraries and characterized 26 potential miRNAs belonging to 21 families in French bean. A total of 140 annotated potential targets were found, of which majority were transcription factors (MYB, bHLH, GRF1, bZIP, NAC etc.,) which may play an important role in stress resistance. RT-qPCR and Northern blot analysis revealed differential expressions of candidate miRNAs and their target genes. The observed induction of miRNA expression is correlated with the down regulation of their targets. Investigation of gene ontology linked with targets of miRNAs forecasted their involvement in various biological functions. We anticipate the further studies may offer new avenues in developing stress tolerant variety of French bean.
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