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The objective of this study was to evaluate differences between the red onion cultivar and breeding line using models based on selected fluorescence spectroscopic data built using machine-learning algorithms from different groups of Trees, Functions, Bayes, Meta, Rules, and Lazy. The combination of fluorescence spectroscopy and machine learning is an original approach to the non-destructive and objective discrimination of red onion samples. The selected fluorescence spectroscopic data were used to build models using algorithms from the groups of Trees, Functions, Bayes, Meta, Rules, and Lazy. The most satisfactory results were obtained using J48 and LMT (Logistic Model Tree) from the group of Trees, Multilayer Perceptron, and QDA (Quadratic Discriminant Analysis) from Functions, Naive Bayes from Bayes, Logit Boost from Meta, JRip from Rules, and LWL (Locally Weighted Learning) from Lazy. The average accuracy of discrimination of onion bulbs belonging to ‘Asenovgradska kaba’ and a red breeding line equal to 100% was found in the case of models developed using the LMT, Multilayer Perceptron, Naive Bayes, Logit Boost, and LWL algorithms. The TPR (True Positive Rate), Precision, and F-Measure of 1.000 and FPR (False Positive Rate) of 0.000, as well as the Kappa statistic of 1.0, were determined. The results revealed the usefulness of the approach combining fluorescence spectroscopy and machine learning to distinguish red onion cultivars and breeding lines.

Artificial-intelligence-based analysis methods can provide objective and accurate results. This study aimed to evaluate the performance of machine learning algorithms to classify yeast-inoculated and uninoculated tomato samples using fluorescent spectroscopic data. For this purpose, three different tomato types were used: ‘local dwarf’, ‘Picador’, and ‘Ideal’. Discrimination analysis was applied with six different machine learning (ML) algorithms. Confusion matrices, average accuracies, F-Measure, Precision, ROC (receiver operating characteristic) Area, MCC (Matthews Correlation Coefficient), and precision-recall area values obtained as a result of the application of different ML algorithms were compared. Based on the fluorescence spectroscopic data, the application of six ML algorithms showed that the first two tomato types were classified with 100% accuracy and the last type was classified with 95% accuracy. The results of the study show that the fluorescence spectroscopy data are strongly representative of tomato species. ML methods fed with these data provide high-performance discrimination.