BACKGROUND: A fundamental step in assessing the viability of a CO 2 biofixation system based on microalgae is to identify the maximum CO 2 biofixation yield that can be achieved for this microorganism when it is cultivated under optimum operational growth conditions. Response surface methodology was applied to determine optimum culture conditions for CO 2 biofixation by a recently isolated freshwater cyanobacterium Synechocystis sp. The strain was cultivated in a 1 L bubble column photobioreactor, in semicontinuous mode.
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