The objectives of this study were to isolate and determine the ability of bacterial strains to decompose sugarcane leaves from acid sulfate soils in the Mekong River Delta (MRD). Soil samples were collected from three acid sulfate soils in Ben Luc district, Long An province, Phung Hiep district, Hau Giang province, and Hon Dat district, Kien Giang province where large sugarcane areas cultivated. Six soil samples were collected and coded: LA1, LA2, HG1, HG2, KG1, and KG2. The results of the study have isolated 18 strains of bacteria are capable of producing cellulase enzyme that breaks down cellulose including LA2-4b, LA2-1, LA2-4a, LA2-2, KG2-1, KG2-2a, KG2-2b, KG2-3, KG2-20, KG2-21, KG2-22, KG2-24, KG2-26, KG2-27, LA1-1, LA1-2, LA1-3, LA1-7. All isolated bacterial strains have the ability to effectively decompose sugarcane leaves, have the potential to apply in practical production, and contribute to improving the quality of acid sulfate soils in the MRD. In which, five selected bacterial strains (LA1-1, LA2-4a, LA2-4b, KG2-2b, and KG2-24) were significantly higher (p<0.05) in the decomposition of sugarcane leaves than the treatment without bacteria.