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This work evaluated the effect of dietary supplementation of probiotic, Pediococcus acidilactici and nucleotide (combined or individual) on reproductive performance including semen quality (motility and density) and egg indices (egg diameter, ovum diameter, absolute fecundity, relative fecundity, gonadosomatic index, hepatosomatic index, fertilization rate, and hatching rate) in goldfish (Carassius auratus). Fish (46.9 ± 2.15 g) were acclimatized and divided into eight experimental diets supplemented with P. acidilactici different concentrations (0.1, 0.2, and 0.3% diet) and nucleotides (0 and 0.5% diet) for 180 days. Female fish fed experimental diets showed significant differences in reproductive parameters as compared to control diet (P < 0.05). Combined diet (probiotic 0.2% and nucleotide) had the highest percentage and duration of sperm motility, absolute fecundity, and fertilization success as compared to other diets (P < 0.05). The significance of the results obtained herein underlines the importance of diet in the reproductive processes, supporting the hypothesis that feed additives could improve gamete quality.

This study investigated the effect of dietary supplementation of probiotic Pedicoccus acidilactici and nucleotide (separately or combined) on growth performance, intestinal microbiota, hemato-immunological parameters, and immunity response in goldfish (Carassius auratus). Fish (average weight 5-6 g) were acclimatized and divided into eight experimental diets supplemented with P. acidilactici of different concentrations (0.1, 0.2, and 0.3% diet) and nucleotides (0 and 0.5% diet) for 6 months. Fish fed with experimental diets showed significant differences in terms of final weight, weight gain, feed conversion ratio, daily growth rate, and condition factor when compared to control diet (P < 0.05). Fish fed with probiotic (0.3%) separately and combined with nucleotide (0.5%) had highest RBC and WBC when compared to other diets (P < 0.05), while the highest values for Hb and Hct as well as total protein, glucose, albumin, and globulin were observed in probiotic (0.2%) and nucleotide (0.5%) combined diet. Serum lysozyme and anti-protease activities were significantly higher in probiotic (0.1 and 0.2%) and nucleotide (0.5%) combined diets. Similarly, these two diets combined showed the highest colonization of P. acidilactici when compared to other diets. In conclusion, combined dietary probiotic and nucleotide improve the growth performance, hemato-biochemical parameters, and intestine growth in C. auratus.

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