The present study evaluated the effect of aflatoxin B1 (AFB1) and fumonisin B1 (FB1) either alone, or in association, on rat primary hepatocyte cultures. Cell viability was assessed by flow cytometry after propidium iodine intercalation. DNA fragmentation and apoptosis were assessed by agarose gel electrophoresis and acridine orange and ethidium bromide staining. At the concentrations of AFB1 and FB1 used, the toxins did not decrease cell viability, but did induce apoptosis in a concentration and time-dependent manner.
A B S T R A C TThe study was conducted in a greenhouse at the Agricultural Research Corporation of Rio Grande do Norte (EMPARN), in order to evaluate the effect of biofertilizers and potassium phosphate rocks, triple superphosphate (TSP) and potassium chloride (KCl), crushed rocks on the chemical properties of the soil and performance of the bacteria Paenibacillus polymyxa in the absorption of macronutrients by the cowpea bean crop. An Ultisol (10 kg per pot) was used with the addition of biofertilizers at levels 40, 70, 100 and 200% of recommendation for TSP and KCl, inoculated or not with bacteria. There was an effect of fertilization on the absorption of macronutrients, being the best results for P and K with TSP + KCL, and N, Ca and Mg for the biofertilizers. In the chemical properties of the soil, the pH was lower in biofertilizers of higher levels, but it was better especially with its addition to P (BPK200). For Ca it was better with TSP + KCl, and Mg with pure rock. The P. polymyxa did not influence in absorption of elements by the plant. The biofertilizers and rocks could satisfy the nutrient needs of the plants by making them potential for sustainable agriculture.Biofertilizantes e performance do Paenibacillus na absorção de macronutrientes pelo feijão caupi e fertilidade do solo R E S U M O O trabalho foi conduzido em casa de vegetação, na Empresa de Pesquisa Agropecuária do Rio Grande do Norte (EMPARN) para avaliar o efeito de biofertilizantes de rochas potássica e fosfatadas, superfosfato triplo (SFT) e cloreto de potássio (KCl), rochas moídas nas propriedades químicas do solo e no desempenho da bactéria Paenibacillus polymyxa na absorção de macronutrientes na cultura do feijão caupi. Utilizaram-se 10 kg de um Argissolo Amarelo Distrófico por vaso com adição dos biofertilizantes nos níveis 40, 70, 100 e 200% da recomendação para SFT e KCl, inoculados ou não com a bactéria. Houve efeito da fertilização na absorção dos macronutrientes sendo os melhores resultados para P e K com SFT+KCL, e N, Ca e Mg para os biofertilizantes. Nas propriedades químicas do solo o pH foi mais reduzido nos biofertilizantes de maiores níveis porém melhorando com sua adição especialmente para P (BPK200). Para Ca foi melhor com o SFT+KCl, e para Mg rocha pura. O P. polymyxa não influenciou na absorção dos elementos na planta. Os biofertilizantes e as rochas supriram a necessidade de nutrientes das plantas revelando-se como potencial para uma agricultura sustentável.
Key words:PRPG rock powder phosphorus potassium Palavras-chave: RPCP pó de rocha fósforo potássio
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