Different plastic types considered as compostable are found on the market such as petro-based (e.g., polybutylene adipate terephthalate (PBAT)) or bio-based plastics (e.g., polylactic acid, (PLA)). Even if their degradation has been confirmed in industrial compost conditions, investigation of their degradation in natural marine environment has been limited. To better understand biodegradation into natural marine environment, commercial compostable (PBAT, semi-crystalline and amorphous PLA) and non-compostable polymers (low density polyethylene, polystyrene, polyethylene terephthalate, polyvinyl chloride) were submerged in situ on the sediment and in the water column in the Mediterranean Sea. These samples were studied by chemical and microbiological approaches. After 82 days of immersion, no significant bacterial degradation of the different polymers was observed, except some abiotic alterations of PBAT and LDPE probably due to a photooxidation process. However, after 80 days in an enrichment culture containing plastic films as a main carbon source, Marinomonas genus was specifically selected on the PBAT and a weight loss of 12% was highlighted. A better understanding of the bacterial community colonizing these plastics is essential for an eco-design of new biodegradable polymers to allow a rapid degradation in aquatic environment.
Water-soluble
π-conjugated polymers are increasingly envisioned
in biosensors, in which their unique optical and electronic properties
permit a highly sensitive detection of biomolecular targets. In particular,
cationic π-conjugated polymers are attractive for DNA sensing
technologies, through the use of the fluorescence signals either in
physiological solutions or in thin films. However, in the context
of enzymatic activity assays, fluorescence-based methods require covalently
labeling DNA with a dye or an antibody and are limited to short time
scale due to dye photobleaching. In this frame, we report here a
novel possible approach to probe the cleavage of DNA by a restriction
enzyme, in continuous and without covalently labeled DNA substrate.
This is achieved by exploiting unique chiroptical signals arising
from the chiral induction of DNA to a poly[3-(6′-(trimethylphosphonium)hexyl)thiophene-2,5-diyl]
upon interaction. The cleavage of DNA by HpaI, an
endonuclease enzyme, is monitored through circular dichroism (CD)
signals in the spectral range where the polymer absorbs light, i.e.,
far away from the spectral ranges of both DNA and the enzyme. We compare
the results to a conventional noncontinuous assay by polyacrylamide
gel electrophoresis, and we demonstrate that induced CD signals are
effective in probing the enzymatic activity. By means of molecular
dynamics simulations and calculations of CD spectra, we bring molecular
insights into the structure of DNA/polymer supramolecular complexes
before and after the cleavage of DNA. We show that the cleavage of
DNA modifies the dynamics and the organization of the polymer backbone
induced by the DNA helix. Altogether, our results provide detailed
spectroscopic and structural insights into the enzymatic cleavage
of DNA in interaction with a π-conjugated polymer, which could
be helpful for developing chiroptical detection tools to monitor the
catalytic activity in real time.
Anthropogenic metal contamination results in long-term environmental selective pressure with unclear impacts on bacterial communities, which comprise key players in ecosystem functioning. Since metal contamination poses serious toxicity and bioaccumulation issues, assessing their impact on environmental microbiomes is important to respond to current environmental and health issues. Despite elevated metal concentrations, the river sedimentary microbiome near the MetalEurop foundry (France) shows unexpected higher diversity compared with the upstream control site. In this work, a follow-up of the microbial community assembly during a metal contamination event was performed in microcosms with periodic renewal of the supernatant river water. Sediments of the control site were gradually exposed to a mixture of metals (Cd, Cu, Pb and Zn) in order to reach similar concentrations to MetalEurop sediments. Illumina sequencing of 16S rRNA gene amplicons was performed. Metal-resistant genes, czcA and pbrA, as well as IncP plasmid content, were assessed by quantitative PCR. The outcomes of this study support previous in situ observations showing that metals act as community assembly managers, increasing diversity. This work revealed progressive adaptation of the sediment microbiome through the selection of different metal-resistant mechanisms and cross-species interactions involving public good-providing bacteria co-occurring with the rest of the community.
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