Cancer stem cells (CSCs) are a small part of the heterogeneous tumor cell population possessing self-renewal and multilineage differentiation potential as well as a great ability to sustain tumorigenesis. The molecular pathways underlying CSC phenotype are not yet well characterized. MicroRNAs (miRs) are small noncoding RNAs that play a powerful role in biological processes. Early studies have linked miRs to the control of self-renewal and differentiation in normal and cancer stem cells. We aimed to study the functional role of miRs in human breast cancer stem cells (BCSCs), also named mammospheres. We found that miR-221 was upregulated in BCSCs compared to their differentiated counterpart. Similarly, mammospheres from T47D cells had an increased level of miR-221 compared to differentiated cells. Transfection of miR-221 in T47D cells increased the number of mammospheres and the expression of stem cell markers. Among miR-221's targets, we identified DNMT3b. Furthermore, in BCSCs we found that DNMT3b repressed the expression of various stemness genes, such as Nanog and Oct 3/4, acting on the methylation of their promoters, partially reverting the effect of miR-221 on stemness. We hypothesize that miR-221 contributes to breast cancer tumorigenicity by regulating stemness, at least in part through the control of DNMT3b expression.

ABSTRACT:The combined use of high-resolution digital images taken from ground as well as from RPAS (Remotely Piloted Aircraft Systems) have significantly increased the potential of close range digital photogrammetry applications in Cultural Heritage surveying and modeling. It is in fact possible, thanks to SfM (Structure from Motion), to simultaneously process great numbers of aerial and terrestrial images for the production of a dense point cloud of an object. In order to analyze the accuracy of results, we started numerous tests based on the comparison between 3D digital models of a monumental complex realized by the integration of aerial and terrestrial photogrammetry and an accurate TLS (Terrestrial Laser Scanner) reference model of the same object. A lot of digital images of a renaissance castle, assumed as test site, have been taken both by ground level and by RPAS at different distances and flight altitudes and with different flight patterns. As first step of the experimentation, the images were previously processed with Agisoft PhotoScan, one of the most popular photogrammetric software. The comparison between the photogrammetric DSM of the monument and a TLS reference one was carried out by evaluating the average deviation between the points belonging to the two entities, both globally and locally, on individual façades and architectural elements (sections and particular). In this paper the results of the first test are presented. A good agreement between photogrammetric and TLS digital models of the castle is pointed out.

No abstract

ABSTRACT:In order to analyze the potential as well as the limitations of low-cost RPAS photogrammetric systems for architectural cultural heritage reconstruction, some tests were performed by a small RPAS equipped with an ultralight camera. The tests were carried out in a site of remarkable historical interest. A great amount of images were taken with camera's optical axis in vertical and oblique position. Images were processed by the commercial software PhotoScan of Agisoft and numerous models were realized, each of them was compared with an accurate TLS model used as a reference. The test, despite some problems found, has provided good results in terms of accuracy (average error <2cm) and reliability.

ABSTRACT:The balance of a coastal environment is particularly complex: the continuous formation of dunes, their destruction as a result of violent storms, the growth of vegetation and the consequent growth of the dunes themselves are phenomena that significantly affect this balance. This work presents an approach to the long-term monitoring of a complex dune system by means of Unmanned Aerial Vehicles (UAVs). Four different surveys were carried out between November 2015 and November 2016. Aerial photogrammetric data were acquired during flights by a DJI Phantom 2 and a DJI Phantom 3 with cameras in a nadiral arrangement. GNSS receivers in Network Real Time Kinematic (NRTK) mode were used to frame models in the European Terrestrial Reference System. Processing of the captured images consisted in reconstruction of a three-dimensional model using the principles of Structure from Motion (SfM). Particular care was necessary due to the vegetation: filtering of the dense cloud, mainly based on slope detection, was performed to minimize this issue. Final products of the SfM approach were represented by Digital Elevation Models (DEMs) of the sandy coastal environment. Each model was validated by comparison through specially surveyed points. Other analyses were also performed, such as cross sections and computing elevation variations over time. The use of digital photogrammetry by UAVs is particularly reliable: fast acquisition of the images, reconstruction of high-density point clouds, high resolution of final elevation models, as well as flexibility, low cost and accuracy comparable with other available techniques.

The survey of historical façades contains several bottlenecks, mainly related to the geometrical structure, the decorative framework, the presence of natural or artificial obstacles, the environment limitations. Urban context presents additional restrictions, binding by ground acquisition activity and leading to building data loss. The integration of TLS and close-range photogrammetry allows to go over such stuff, not overcoming the shadows effect due to the ground point of view. In the last year the massive use of UAVs in survey activity has permitted to enlarge survey capabilities, reaching a deeper knowledge in the architecture analysis. In the meanwhile, several behaviour rules have been introduced in different countries, regulating the UAVs use in different field, strongly restricting their application in urban areas. Recently very small and light platforms have been presented, which can partially overcome these rules restrictions, opening to very interesting future scenarios. This article presents the application of one of these very small RPAS (less than 300&amp;thinsp;g), equipped with a low-cost camera, in a close range photogrammetric survey of an historical building façade in Bologna (Italy). The suggested analysis tries to point out the system accuracy and details acquisition capacity. The final aim of the paper is to validate the application of this new platform in an architectonic survey pipeline, widening the future application of close-range photogrammetry in the architecture acquisition process.

No abstract

The Amalfi Coast represents a unicum in which the complexity is due to the coexistence of natural singularities and multiple traces resulting from the adaptation made by man to the harsh orography and to the scarcity of resources. Included in the World Heritage List in 1997, the area of the UNESCO perimeter is placed at the centre of the programmatic guidelines of the Management Plan which articulates the objectives around the themes of knowledge, protection, conservation, enhancement, and communication of (and for) coastal cultural heritage. Given these premises, the contribution aims to present a critical balance between the prospects for intervention and the operations concretely defined for the conservation of the cultural landscape of the Amalfi Coast, paying attention to the fate of the proto-industrial heritage (paper mills, ironworks, mills, and hydraulic works), largely abandoned and which requires targeted strategies for the recognition of specificities and the mitigation of intrinsic vulnerabilities.