The method of Non-equilibrium Energy Spectrum (NES) was applied in measurement of hydrogen bonds energy distribution in 1% Rosa damscena L. oil solution in deionized water. Local maxima in this spectrum were identical with these obtained in investigations of other biologically active solutions and related to particular bio effects as follows: (-0.1387 eV; 8.95 µm; 1117 cm-1). This local maximum is typical for antibacterial, anti-tumor and anti-inflammatory effects. The local maxima at (-0.1212 eV; 10.23 µm; 978 cm-1) and (-0.1262 eV; 9.82 µm; 1018 cm-1) are typical for anti-inflammatory effects and this at (-0.1112 eV; 11.15 µm; 897 cm-1) is typical for effects on the nervous system and nerve conductivity. Information theoretical analysis was performed using the values of Shannon entropy and Transformational information entropy, pointing to hydrogen bonds distribution similarities between Rosa damscena L., V. myrtillus L. and Salvia divinorum Epling. The possible chemical causes of these similarities were identified as antioxidant activity and polyphenols concentration.
A study was performed with the methods of Non-equilibrium energy spectrum (NES), Differential non-equilibrium energy spectrum (DNES), mathematical models and water cluster analysis on the changes of hydrogen bonds energy distribution in aqueous solutions due to calcium ions (Ca2+) from the medicinal plants: Origanum vulgare L., Thymus vulgaris L., Mentha longifolia L., Petroselinum crispum Mill., Sideritis scardica Griseb., Salvia officinalis L. All of them are known to contain more than 1000 mg/100 g of calcium ions. The local maximum at (E = -0.1112 eV) (? = 11.15 ?m) (? = 897 cm-1) in all investigated solutions was associated with the presence of calcium ions by Student’s t-test at P<0,05. In the proposed practical approach, the solutions had similar clustering patterns at E = -0.1387 eV, corresponding to water clusters of 16-20 water molecules. These findings from different plant species further expand the evidence about the specific influence of calcium ions on the -0.1112 eV hydrogen bonds energy level in aqueous solutions.
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