The intensive use of pesticides in agriculture has prompted researchers to develop new methods for identifying these pollutants in water. This study sought to validate a high performance liquid chromatography (HPLC) method to determine the concentration of the pesticide glyphosate in groundwater samples by using solid-phase extraction (SPE) filters after derivatization with chloroformate 9-fluorenylmethoxycarbonyl (FMOC-Cl). For the HPLC method, we evaluated the following main validation parameters: linearity, specificity, precision, accuracy, robustness, and limits of detection and quantification. After validation of the method, we determined the concentration of glyphosate in samples from thirteen deep, tubular wells distributed in urban and rural areas in Chapecó, SC, Brazil. The solvent used in the extraction of excess FMOC-Cl was dichloromethane and subsequently filtration was performed on C18 SPE, and injected into the chromatograph column in amino polymer with fluorescence detection. The analytical curve made in ultrapure water was linear, with a correlation coefficient of 0.99. The limits of detection and quantification were 0.24 and 0.07 µg L-1 , respectively. Recovery tests in natural waters ranged from 90.37 to 101.70%. Glyphosate was detected in 5 of the thirteen wells evaluated. The highest concentration of glyphosate (6.80 µg L-1) was detected in a countryside well, near the municipal water supply. Despite the low levels of glyphosate detected in our study, any amount present in groundwater samples is worrisome, as these molecules have low ground mobility.
Integrated management of municipal solid waste seeks sustainability under the premises of environmental protection, health promotion, and economic development. In this context, this article proposes actions and policy tools for local governments to improve quality and governance in waste management. A set of 23 actions is proposed based on existing conceptual models and available legislation. The results are presented in three levels as follows: planning of the administrative and operational structure, waste management, and performance evaluation. The results showed that the proposed actions need to be implemented through policy tools, such as municipal legislation, social communication, normative instructions, and technical studies. The applicability of the proposed methodology, which might be replicated in any municipality to increase the efficiency of the waste management system, was positively evaluated in a medium-sized Brazilian city, which presented an overall attendance level of 52%.
Among several pesticides used in agriculture, glyphosate herbicide is one of the most employed in crop fields in spite of its recognized toxic effects on human health and on the environment. Consecutive use of glyphosate in crops can cause significantly increase the severity of various diseases, reduced growth, impaired defenses and alter the physiology of plants affecting the susceptibility or tolerance to diseases. Peroxidase enzymes play an important role in some physiological and pathological processes, involving active oxygen species occurring at the level of plasma membrane and cell wall. The aim of this work was to evaluate the effect of glyphosate on the enzymatic activity of horseradish peroxidase. For this purpose, tests were conducted to determine the optimal pH range and then reaction kinetic where was added, the substrate (catechol), mediator (H 2 O 2), glyphosate and enzyme (horseradish peroxidase), varying the substrate concentration glyphosate to assess the activity of this reaction and the influence of herbicide on the enzyme activity. Results demonstrate that glyphosate increases the activity of horseradish peroxidase in low concentration and can decrease the resistance of the plant to the entry of pathogens.
Direitos para esta edição cedidos à Atena Editora pelos autores. Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição-Não-Comercial-NãoDerivativos 4.0 Internacional (CC BY-NC-ND 4.0).O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores, inclusive não representam necessariamente a posição oficial da Atena Editora. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais. Todos os manuscritos foram previamente submetidos à avaliação cega pelos pares, membros do Conselho Editorial desta Editora, tendo sido aprovados para a publicação com base em critérios de neutralidade e imparcialidade acadêmica.A Atena Editora é comprometida em garantir a integridade editorial em todas as etapas do processo de publicação, evitando plágio, dados ou resultados fraudulentos e impedindo que interesses financeiros comprometam os padrões éticos da publicação. Situações suspeitas de má conduta científica serão investigadas sob o mais alto padrão de rigor acadêmico e ético.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.