Background:Two of the most critical factors affecting the prognosis of an avulsed tooth after replantation are extra oral dry time and the storage medium in which the tooth is placed before treatment is rendered. However, the ability of a storage/transport medium to support cell viability can be more important than the extra oral time to prevent ankylosis and replacement resorption.Aim:Purpose of this study was evaluation and comparison of efficacy of a new storage medium, oral rehydration solution (ORS) with coconut water, and propolis in maintaining the viability of periodontal ligament (PDL) cells by using a collagenase-dispase assay.Materials and Methods:40 teeth were selected with intact crown which were advised for Orthodontic extraction having healthy PDL. Teeth were then randomly divided into three experimental storage solution groups. Other 10 were divided into positive and negative control groups (5 each).Statistical Analysis and Result:The results were statistically analyzed with analysis of variance and multiple range by using post hoc tests. The results of the prevailing study indicated that coconut water group demonstrated a significantly higher number of viable PDL cells than propolis 50%, and ORS. There was no significant difference between coconut water and propolis 50% groups.

Background:Micro-organisms are the primary causative agents of endodontic infections. Phenotype based procedures for bacterial identification has certain drawbacks especially, when investigating the microbiota of root-filled teeth. Thus, more sensitive methods like Polymerase chain reaction (PCR) can provide results that are more accurate and reliable for the microbial prevalence in the root filled teeth.Aim:In this study, we have investigated twenty symptomatic root-filled teeth with chronic apical periodontitis for the prevalence of Enterococcus faecalis and Candida albicans in the root filled teeth associated with symptomatic cases with or without periradicular lesions.Materials and Methods:Microbiological samples were taken from the canals immediately after removal of previous gutta percha cones using aseptic techniques. After removal of root canal filling, samples were obtained with paper points placed in the canal. Paper points were transferred to a cryotube containing “Tris EDTA” buffer and immediately frozen at −20°C.Results:By PCR amplification of the samples using taxon specific primers, E. faecalis was found to be prevalent species, detected in 65% of the cases and C. albicans was detected in 35% of cases.Conclusion:The results of the study shows that geographical influence and dietary factors might have some role to play in the prevalence of the species like C. albicans and presence of E. faecalis confirming the assertion of previous culture-dependent and independent approaches for the microbiological survey of root filled teeth.

Abstract. The complexity of human oral functional movements has not been studied in detail quantitatively, and only recently have studies begun to evaluate whether such movements contain sex-specific characteristics. Therefore, the purposes of this study were: (1) to quantify in detail the jaw movements and associated masticatory electromyographic activity occurring during gum chewing, and (2) to explore these data for evidence of sex specificity. Fourteen male and 17 female subjects participated in the study. Approximately 11 right-and 11 left-sided chewing cycles and associated masticatory electromyographic activity were sampled from each subject. The samples were quantified into 165 variables per chewing cycle, averaged to create a single multivariate vector for each subject, and then analyzed by a step-wise discriminant analysis. With a combination of 6 variables, a jackknifed cross-validation test found the probability of correct classification to be 93.5%. These findings support the hypothesis that masticatory jaw movements contain sex-specific features.