The current investigation focuses on developing a reproducible protocol for callus induction and for studying the precursor L-phenylalanine impact on product enhancement for alkaloid production and measurement of pH. Alkaloids are nitrogen-containing compounds; individual alkaloids were purified and synthesized commercially as excellent substances with recognized and experimentally verified pharmacological attributes because of their antimicrobial, anticancer properties and other therapeutic values. The current studies explore the impact of Precursor on alkaloid production by cell suspension culture in G. glauca, resulting in an increase in the cell growth observed and quantified by HPLC, TLC analysis, and the presence of functional groups identified by FTIR profiling. Additionally, this finding offers a protocol for biomass production of alkaloids from medicinally important endangered plants aiding in the conservation of the plants as well as producing commercially valuable secondary metabolites.
Phytocompounds enable plants to play a remarkable role as traditional remedies for human ailments. Due to their extremely low side effects, the ancient herbal medicinal systems are more beneficial than the modern medical systems. Gnidia glauca (Fresen.) Gilg has many biological properties, including antifungal, anticancer, antibacterial, and traditional medicine for swelling, indigestion, and snake bites. Because of the plant's impressive qualities and status as an endangered plant species, the callus suspension method was used as an alternative technique for phytocompounds enhancement. In order to screen the presence of phytocompounds, qualitative estimation was used, and ten phytoconstituents were identified by Gas Chromatography-Mass Spectroscopy (GC-MS) analysis in the cell suspension extracts. The present study's findings highlight the need for more investigations into the large-scale production and purification of bioactive substances.
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