The environmental effects of adding certain selected petroleum products to field soils at widely separated geographical locations under optimum conditions for biodegradation were studied. The locations selected for study of soil biodegradation of six oils (used crankcase oil from cars, used crankcase oil from trucks, an Arabian Heavy crude oil, a Coastal Mix crude oil, a home heating oil no. 2, and a residual fuel oil no. 6) were Marcus Hook, Pennsylvania, Tulsa, Oklahoma, and Corpus Christi, Texas. The investigative process, covering a period of 1 year at each location, was conducted in 14 fields plots (1.7 by 3.0 m) to which
A soil isolate identified as a strain of Nocardia corallina accumulated a , a'dimethyl-cis, cis-muconic acid under co-oxidation conditions employing n-hexadecane for growth and p-xylene as the co-oxidizable substrate. N. coraifina V-49 was postulated to have two pathways for the oxidation of p-xylene. One pathway proceeds through p-benzyl alcohol, p-tolualdehyde, and p-toluic acid to 2,3dihydroxy-p-toluic acid, and the other pathway results in ortho ring cleavage of 3,6-dimethylpyrocatechol and hence accumulation of a,a'-dimethyl-cis,cismuconic acid.
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