Б а с р е д а к т о р ы х. ғ. д., проф., ҚР ҰҒА академигі М. Ж. Жұрынов Р е д а к ц и я а л қ а с ы:Ресей) Абишев М.Е. проф., корр.-мүшесі (Қазақстан) Аврамов К.В. проф. (Украина) Аппель Юрген проф. (Германия) Баймуқанов Д.А. проф., корр.-мүшесі (Қазақстан) Байтулин И.О. проф., академик (Қазақстан) Банас Иозеф проф. (Польша) Берсимбаев Р.И. проф., академик (Қазақстан) Велесько С. проф. (Германия) Велихов Е.П. проф., РҒА академигі (Ресей) Гашимзаде Ф. проф., академик (Əзірбайжан) Гончарук В.В. проф., академик (Украина) Давлетов А.Е. проф., корр.-мүшесі (Қазақстан) Джрбашян Р.Т. проф., академик (Армения) Қалимолдаев М.Н. проф., академик (Қазақстан), бас ред. орынбасары Лаверов Н.П. проф., академик РАН (Россия) Лупашку Ф. проф., корр.-мүшесі (Молдова) Мохд Хасан Селамат проф. (Малайзия) Мырхалықов Ж.У. проф., академик (Қазақстан) Новак Изабелла проф. (Польша) Огарь Н.П. проф., корр.-мүшесі (Қазақстан) Полещук О.Х. проф. (Ресей) Поняев А.И. проф. (Ресей) Сагиян А.С. проф., академик (Армения) Сатубалдин С.С. проф., академик (Қазақстан) Таткеева Г.Г. проф., корр.-мүшесі (Қазақстан) Умбетаев И. проф., академик (Қазақстан) Хрипунов Г.С. проф. (Украина) Юлдашбаев Ю.А. проф., РҒА корр-мүшесі (Ресей) Якубова М.М. проф., академик (Тəжікстан) «Қазақстан Республикасы Ұлттық ғылым академиясының Хабаршысы».
The analysis of the dynamics of changes in serological and hematological blood parameters of cattle over the period of comprehensive healthrelated measures against Bovine Leukemia Virus (BLV) infection is presented to assess their effectiveness. For analysis of the epizootological situation, veterinary reporting data and the results of serological and hematological diagnostics for cattle leukemia were used. The work was performed in three agricultural enterprises of the Siberian Federal District: Irkutsk Region Altai Territory and Novosibirsk Region. According to the principle of an individual approach, a programme of comprehensive diagnostic and health anti-leukemia measures for each enterprise was developed. The main purpose of these measures was to break the epizootic chain in chronic BLV infection and ultimately change the epizootic situation by reducing the percentage of infection in the livestock population. The positive dynamics of the reduction of infected and leukemiasick cattle was shown as part of a complex of antiepizootic measures, which was made possible by a deviation from the generally accepted regulations for serological diagnostics in agar gel (AGID). During the quarterly diagnosis, the infection rate decreased over 2 years from 72.41 to 1.94% in cows and from 66.6% to negative results in heifers of the breeding age. Using a more sensitive method of enzymelinked immunosorbent assay in the recovery of young and repair livestock, it was possible to reduce infection from 20.9 to 4.0% and from 43.0 to 2.7%, respectively. In this case the epizootic situation for BLV infection may be more stable due to the identifi cation of hidden carriers of the infection.
In recent years, using a genome-wide association study (GWAS), a number of single nucleotide polymorphisms (SNPs) have been suggested to be associated with susceptibility to leukemia in cattle. However, all studies have been done with purebred Holstein cows and their hybrids. In this regard, it is important to confirm the functional role of polymorphisms previously identified in a GWAS study in Russian cattle breeds. The aim of this study was to verify the association between rs110861313 in the intergenic region of bovine chromosome 23 and leukemia in the Russian Black Pied cattle. Based on the levels of bovine leukemia virus (BLV)-specific antibodies detected in serum using serodiagnostic techniques, animals were divided into three groups: healthy animals (n = 115), asymptomatic virus carriers (n = 145) and animals with leukemia (n = 107). Genotyping of rs110861313 was carried out using polymerase chain reaction followed by analysis of restriction fragment length polymorphisms. A significant decrease in the frequency of the A/A genotype (11.2 %) was revealed in animals with persistent lymphocytosis compared to virus carriers (27.6 %) (p < 0.002). At the same time, the frequency of animals with the C/C genotype in animals with persistent lymphocytosis (41.1 %) was significantly higher than that of virus carriers (21.4 %) (p < 0.001). In this case, asymptomatic virus carriers can be considered a more suitable control than healthy animals that have not been in contact with the virus. According to bioinformatics analysis, resistance to BLV can be due to the presence of the transcription factor FOXM1 binding site in the region of rs110861313. FOXM1 is expressed in immune cells and can potentially affect the expression of the neighboring genes (LY6G5B, GPANK1, ABHD16A, LY6G6F, LY6G6E, CSNK2B, ApoM). Thus, we found that SNP rs110861313 in the intergenic region of bovine chromosome 23 is associated with the development of leukemia following BLV infection in the Russian Black Pied cattle.
The analysis was carried out on the severity of the epizootic situation on leukemia and the presence of risk factors for the prevalence of this infection in 67 farms of Novosibirsk region, unfavorable for leukemia. Ten risk factors which can be attributed to the exogenous category of factors (technological, iatrogenic) were chosen as the subject of this study. These risk factors can have the greatest impact on the severity of the epizootic process in leukemia infection. The qualitative and quantitative representations of risk factors in the farms were analyzed, the most frequently registered of them were determined. The correlation between individual risk factors and such indicators as the level of leukemia incidence in cows and the infection rate of cows and heifers was assessed. It was established that there is a correlation between the leukemia incidence in cows and the following factors: the use of calves from cows with hematological diseases (p < 0.001) for reproduction of the herd; the absence of regular monitoring of the serological status of cows for BLV-infection (p < 0.001); the lack of regular monitoring of the serological status of cows for leukemia (p < 0.001); the use of natural insemination (p < 0.001). It was also identifi ed that there is a correlation between infection of heifers and joint maintenance and grazing of cattle with different status for BLV-infection (p < 0.001).
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