<p>The pro-antioxidant balance in the liver and muscles of sterlet under the influence of artificial carbonic dioxyde hibernation and anaesthesia have been studied. In experiments were used two-years old sterlet weighing 170–210 g which were grown under conditions of a closed water supply. Four groups of five copies of each fish were formed: Control group I (fish remained intact); Group II (the clove oil was used for fish anaesthesia, which was added to the water); Group III (fish were hibernated by carbon dioxide); Group IV - control of hibernation (after complete recovery from carbon dioxide hibernation, these groups of fish were returned to the pool of incubation shop for her follow–up). The research of intensity of the formation products of peroxide oxidation of lipids (POL) in the body of sterlet in the experimental conditions were conducted by determining the content of thiobarbituric acid active products (TBA–active products) in the muscles and liver that are generated at the final stages of lipid peroxidation. The content of malonic dialdehyde (MDA), one of the intermediate products of lipid peroxidation of membrane was determined photometrically by the concentration of colored complex formed by its reaction in the acidic environment of the two molecules of thiobarbituric acid (TBA). Catalase activity was determined by the ability of hydrogen peroxide to form the stable colored complex with Molybdenum salts. The results support the adaptive character in fluctuations in the antioxidant protection system by the actions of carbon dioxide hibernation and anaesthesia on the sterlet body. This also contributes to usage of these conditions in fish transportation at long distances without stress factors.</p>
SECTION 1. BIOLOGY
Correcting effectiveness of milk phospholipides by calves enteropatнologyAn important issue in the current state of the livestock industry is the preservation of the stock of young animals and their productive qualities, the most intensive formation of which occurs in the early postnatal period of life. The applied value of these studies is confirmed by the high percentage of cases of young animals of different species on enteropathology. It is known that 60-90% of newborns are sick, of which 15 to 50% die [1].Numerous studies by employees of the National University of Life and Environmental sciences of Ukraine (NULES of Ukraine) prove that the metabolic status of the body of newborn animals is distinguished by a significant lability of biochemical parameters, which is associated with the normalization of the acid-base state, the genetically determined process of replacing the fetal type of blood proteins with an adult, the formation of a unique natural phenomena -colostral immunity with endocytotic-pinocytotic mechanism of assimilation colostrum immune proteins in the gastrointestinal tract and with a number of features of the morphological and functional condition of the structural elements of cells and, above all, their membranes [2-5].The development of enteropathology in this "critical" period significantly violates the formation of adaptive changes in the digestive tract and other organs and systems of newborns [5, 6]. In these animals, significant metabolic disturbances were noted during their clinical recovery [5, 7]. This indicates deep metabolic disorders at the
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