Synthetic growth stimulants are widely used to get high productivity of animals. These preparations can accumulate in the meat and their residual quantities will adversely affect the health of consumers. The purpose of the work was to monitor the content of zeranol, growth stimulant of ruminants in beef which goes to meat processing enterprises of the Western region of Ukraine and to determine the effect of heat treatment on its quantity. It was found out that 29.8% of beef samples taken at meat processing enterprises contained a stimulant for the growth of ruminant zeranol. It was found that during the storage of beef samples frozen at a temperature of -18 °C with different content of zeranol there is a decrease in its number. The most intense process of destruction of zeranol occurred during the first month of storage, during this period of time the amount of zeranol is reduced by an average of 20%, regardless of the initial content. Within two months of storage of frozen beef, the content of zeranol decreases by 28.2 ±0.17%, and at the end of the sixth month its quantity decreases to 33.2 ±0.58%. It was also found that the dynamics of zeranol reducing in beef samples with large quantities (22.5 μg.kg-1) and small (2.3 μg.kg-1) were the same. It was set up that during 30 min of meat cooking there was a decrease in the content of zeranol 24.7 ±0.23% and 32.0 ±0.35% for 60 min, compared to its content in fresh meat. At the same time, when stored in the frozen state and subsequent cooking, the reduction of zeranol content in meat was 39.3 ±0.3%. Therefore, it is proposed to revise and amendments into the regulatory documents of Ukraine regarding the control and supervision of the presence of hormone (zeranol) residues in meat and meat products in order to prevent their sale and consumption by humans.
The issue of healthy eating has always been relevant, especially this problem has been exacerbated by the rapid development of food technology and the production of a significant number of semi-finished products. As a result, there is a need to develop new types of food, including unleavened and low-calorie bread, bakery products enriched with vitamins, minerals, plant supplements. The aim of the work was to substantiate the technology of bread production with the use of beet kvass and to investigate the physico-chemical quality indicators of experimental samples of finished products. Technological indicators that characterize the quality of bread: moisture, porosity, crumbliness, acidity were determined by generally accepted methods. Bread made with beet kvass has been found to have well-developed crumb porosity, does not crumble for a long time, hardens slowly, and is resistant to microbiological spoilage. The crumb is not deformed and elastic when pressed. Replacing part of the water during the kneading of wheat dough with beet kvass (50 % by weight of flour) makes it possible to intensify the maturation of semi-finished products, both in traditional and accelerated technologies, to create a more complete nutrient medium for yeast activation, to obtain high quality bakery products storage. It has also been found that during the storage of bread, the hydrophilic properties of the crumb, its crumbliness changes slightly. The drying process is most intense during the first day, then slows down.
The bakery industry is increasingly using technologies that improve the quality of finished products. Such technologies of bread production include technology with the use of natural leavens of spontaneous fermentation, which are made without the addition of various artificially created chemical ingredients. The aim of the work was to determine the influence of basil seeds on the activity of rye-wheat leaven in the technology of its renewal and to investigate the physicochemical, organoleptic characteristics of the dough during fermentation and the quality of bread. The research used generally accepted in the bakery industry methods of physical and chemical research of sourdough, dough and finished products. It was found that the introduction of basil seeds in the composition of spontaneous leaven in the amount of 1.5 to 3.0 % reduces the time of renewal of the leaven and accelerate the increase in acidity, which is the result of the development of its own microbiota. The expediency of adding basil seeds (up to 2.0 %) to the technology of spontaneous leaven production was established, as the time of ball emergence after the fifth stage of renewal was 8 minutes shorter compared to the control sample of leaven. The addition of basil accelerates the development and biochemical activity of the yeast microbiota, resulting in more enzymes of the reductase class. The dough is made with the addition of spontaneous fermentation leaven, which contains basil seeds ferment and ripen faster. In the evaluation of bread samples, the highest total score was obtained by samples № 2 and №3, which were made on spontaneous leaven with a content of 1.0 and 1.5 basil – the total number of points was 19.3 points, which is 0.5 points more than the control sample. Therefore, for the production of rye-wheat spontaneous leaven, it is advisable to add basil seeds to its composition to enhance biochemical and microbiological processes.
Unrefrigerated fish and seafood are dominated by mesophilic microflora. At the same time, during their storage in the conditions of refrigerating chambers, a cold-loving – psychrotrophic microbiota dominates, which, according to many scientists, causes organoleptic and chemical changes in fish and affects sanitary and hygienic indicators. Therefore, the microbiological assessment of frozen fish by the content of psychrotrophic microbiota, which participates in its reduced safety and quality, will make it possible to propose preventive measures against spoilage. The aim of the work was to develop a microbiological criterion for the hygiene of the technological process of frozen fish based on the assessment of the content of psychrotrophic microbiota. In the work, standard methods were used, in particular, the microbial number was determined in the samples at a temperature of (30 ± 1) ºС incubation of crops for 72 hours and at a temperature of (6.5 ± 0.5) ºС incubation for 10 days (psychrotrophic microbiota). It was established that during the storage of frozen fish at a temperature of -18 °С, the mesophilic microflora is inactivated, as a result of which its number gradually decreases. However, the intensity of inactivation of mesophilic bacteria in samples with insignificant microbial insemination is, on average, 1.4 times faster, compared to samples with a larger number of microorganisms. In our opinion, this is due to the fact that the composition of mysophilous microflora is largely represented by genera of bacteria that show tolerance to cold. At the same time, the psychrotrophic microflora of frozen fish does not become inactivated during the 8-month storage period at a temperature of -18 °C, and is more stable compared to the mesophilic one. That is, no significant increase or decrease in the number of microorganisms was noted. As a result, by the amount of psychrotrophic microflora of frozen fish, determined at any stage of storage, it is possible to judge its amount at the time of freezing. The amount of psychrotrophic microflora more objectively characterizes the hygienic conditions of production, compliance with the freezing temperature. The microbiological hygiene criteria of the technological process of frozen fish production were substantiated and developed based on the estimation of the amount of psychrotrophic microflora (n = 5; c = 3; m = 10000 CFU/g; M = 50000 CFU/g). These criteria indicate compliance with a set of hygienic measures from freezing to circulation of fish. Therefore, the determined microbiological criterion for the quantity of psychrotrophic microflora in frozen fish complements the existing methods of evaluating the hygiene of the technological process and aims to broadly assess the microbiological safety of frozen fish.
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