Objectives
A previous single‐country pilot study indicated serum anti‐GM2 and anti‐GA1 anti‐glycolipid antibodies as potential biomarkers for acute canine polyradiculoneuritis. This study aims to validate these findings in a large geographically heterogenous cohort.
Materials and Methods
Sera from 175 dogs clinically diagnosed with acute canine polyradiculoneuritis, 112 dogs with other peripheral nerve, cranial nerve or neuromuscular disorders and 226 neurologically normal dogs were screened for anti‐glycolipid antibodies against 11 common glycolipid targets to determine the immunoglobulin G anti‐glycolipid antibodies with the highest combined sensitivity and specificity for acute canine polyradiculoneuritis.
Results
Anti‐GM2 anti‐glycolipid antibodies reached the highest combined sensitivity and specificity (sensitivity: 65.1%, 95% confidence interval 57.6 to 72.2%; specificity: 90.2%, 95% confidence interval 83.1 to 95.0%), followed by anti‐GalNAc‐GD1a anti‐glycolipid antibodies (sensitivity: 61.7%, 95% confidence interval 54.1 to 68.9%; specificity: 89.3%, 95% confidence interval 82.0 to 94.3%) and these anti‐glycolipid antibodies were frequently present concomitantly. Anti‐GA1 anti‐glycolipid antibodies were detected in both acute canine polyradiculoneuritis and control animals. Both for anti‐GM2 and anti‐GalNAc‐GD1a anti‐glycolipid antibodies, sex was found a significantly associated factor with a female to male odds ratio of 2.55 (1.27 to 5.31) and 3.00 (1.22 to 7.89), respectively. Anti‐GalNAc‐GD1a anti‐glycolipid antibodies were more commonly observed in dogs unable to walk (OR 4.56, 1.56 to 14.87).
Clinical Significance
Anti‐GM2 and anti‐GalNAc‐GD1a immunoglobulin G anti‐glycolipid antibodies represent serum biomarkers for acute canine polyradiculoneuritis.
This method of quantifying the T1-weighted magnetic resonance imaging signal intensity of the lentiform nuclei was reliable and showed that dogs with portosystemic shunts tend to have increased signal intensity. Further prospective studies are necessary to investigate the clinical significance and applications of these findings.
Venous air embolism entering via the ventral internal vertebral venous plexus was suspected during thoracic spinal surgery in two dogs. In both cases, air was seen bubbling from a pool of blood on the floor of the vertebral canal accompanied by sudden cardiopulmonary disturbances: low end-tidal carbon dioxide pressure, tachycardia and reduction in oxygen in the blood. One dog became dyspnoeic and one died.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.