Researches for the viral infection in woody plants of Ukraine forest biocenosis were first studied. Sampling was conducted in forests, parks and gardens in the Ukrainian Polissya Area (Zhytomyr, Kyiv region). Visual studies, electron microscopy, ELISA, method of internally cell inclusions, indicator plants, computer support (microscope-object-monitor) are used in work. TMV isolate mainly causes necrosis in Platanus orientalis and induces a kind of mosaic symptoms that turns into chlorosis during July — August in Acer platanoides. Other symptoms are observed such as leaves twisting and dying off, necrosis, wood damage and premature defoliation. It is found that identified TMV isolate has specific serological similarity with viruses of taxonomic groups that affect tomato, tobacco, sunflower seeds, and medical plants such as plantain and hops
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