INTRODUCTION Biosurfactants are potential surfactants obtained from natural sources and gives several advantages over chemically synthesized surfactants. They are less toxic in nature, biodegradable and environmentally acceptable 1) and hence attracted a great attention. SLs, surface-active glycolipids are one of the well-known members of biosurfactant family produced by microorganisms and extracellularly by Candida bombicola 2-5). These are amphiphilic in nature, i.e. containing both hydrophobic as well as hydrophilic moieties making them able to help in reduction of surface and interfacial tension at the surface and interface respectively 6). The physicochemical properties, such as decrease in interfacial tension, heat and pH stability of many biosurfactants have been shown to be comparable to synthetic surfactants 7). These biosurfactants are widely used in the food, pharmaceutical, cosmetic, and cleaning industries. Optimization of media component is one of the most important stages in production of any fermentative product. Single parameter study is time consuming and it will not explain the interactions between independent variables.
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