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We demonstrate a technique for detecting magnetically labeled Listeria monocytogenes and for measuring the binding rate between antibody-linked magnetic particles and bacteria. This sensitive assay quantifies specific bacteria in a sample without the need to immobilize them or wash away unbound magnetic particles. In the measurement, we add 50-nm-diameter superparamagnetic magnetite particles, coated with antibodies, to an aqueous sample containing L. monocytogenes. We apply a pulsed magnetic field to align the magnetic dipole moments and use a hightransition temperature superconducting quantum interference device, an extremely sensitive detector of magnetic flux, to measure the magnetic relaxation signal when the field is turned off. Unbound particles randomize direction by Brownian rotation too quickly to be detected. In contrast, particles bound to L. monocytogenes are effectively immobilized and relax in about 1 s by rotation of the internal dipole moment. This Né el relaxation process is detected by the superconducting quantum interference device. The measurements indicate a detection limit of (5.6 ؎ 1.1) ؋ 10 6 L. monocytogenes in our sample volume of 20 l. If the sample volume were reduced to 1 nl, we estimate that the detection limit could be improved to 230 ؎ 40 L. monocytogenes cells. Timeresolved measurements yield the binding rate between the particles and bacteria.A ntibodies are widely used as biological probes to identify specific microorganisms or molecules (1, 2). The antibodies are linked to a label and introduced into the sample, where they bind to the targets of interest and provide a means of detection. Common labels include enzymes, fluorescent dyes, radioisotopes, or magnetic particles. This general technique has various applications. In an immunoassay, the goal is to detect and quantify specific targets. Tagged antibodies can also be used to separate target antigens selectively or to measure the affinity between antibody and antigen. In this article, we present a sensitive method for detecting magnetically labeled bacteria by using a superconducting quantum interference device (SQUID), a highly sensitive detector of magnetic flux. This assay can be used to monitor bacteria in a liquid sample and to determine the rate of binding between antibody-linked particles and bacteria.Magnetic particles have several advantages as labels. They are stable and nontoxic and can be manipulated with a magnetic field, making it possible to separate target antigens magnetically (3). Methods have been developed to detect small numbers of such particles by using Hall probes (4), giant magnetoresistance arrays (5), atomic force microscopy (6), force-amplified biological sensors (7), and SQUIDs (8-10).Weitschies, Kötitz, and colleagues pioneered the use of SQUIDs for magnetic immunoassays (8,(11)(12)(13)(14)(15)(16). They developed a magnetic relaxation immunoassay in which magnetic particles bound to targets are distinguished from unbound particles by their different relaxation times. By using a low-criticalte...

We investigated the kinetics and composition of the second phase of the wound repair process of Dasycladus vermicularis ([Scropoli] Krasser) using fluorescent probes, chromatography, UV spectroscopy, and histochemistry. Our new evidence supports the hypothesis that the second phase of wound repair (initiated at approximately 35-45 min postinjury) is based on the activation of an oxidative burst that produces micromolar H 2 O 2 levels. These results provide evidence of peroxidase activity at the wound site, real-time measurements of an oxidative burst, and catechol localization in wound plugs. Strong evidence is presented indicating that the biochemical machinery exists for oxidative cross-linking to ensue in the wound-healing process of D. vermicularis.