The aim: To study peculiarities of morphological and immunohistochemical changes of stomach’s mucosa in eosinophilic gastritis in children
Materials and methods: 64.1±6.0% patients with eosinophilic gastritis and 35.9±6.0% patients with lymphocytic gastritis participated in our investigation. In order to verify the diagnosis morphological and immunohistochemical diagnostics of the stomach’s mucosa was performed in all children. To assess morphological changes in tissues the specimens were colored with hematoxylin, eosin and picrofuchsin by van Gieson’s. Indirect streptavidin-peroxydase staining method was used for immunohistochemical investigation and the following indexes were assessed: proliferating cell nuclear antigen – PCNA, Bcl – 2, Вax, Collagen Type ІV, TGFβ and NF-κβ.
Results: Comparative analysis of morphologic investigation has demonstrated that eosinophilic gastritis is characterized by fibrosis and fibroblasts proliferation into basal and superficial parts of mucosa’s lamina propria, multiple hemorrhages, thrombosis and erosions on the background of eosinophilic infiltration. Immunohistochemical indexes of cellular restoration in eosinophilic gastritis are characterized by increased proliferative activity and decreased indexes of proapoptotic and antiapoptotic activity. Prevalence of the reaction with the use of monoclonal antibodies to Collagen Type IV in majority of children with eosinophilic gastritis was characterized by separate fragmented foci in basal membranes of superficial epithelium. Remarkable TGFβ immune coloration was detected in majority of children on the background of fibrosis and eosinophilic infiltration of lamina propria. NF-κβ expression in epitheliocytes’ cytoplasm and nuclei was uneven. Homogenous remarkable coloration was detected in majority of patients with lymphocytic infiltration of mucosa.
Conclusions: Eosinophilic gastritis course in children is characterized by remarkable inflammation, decreased regeneration of the mucosa, impairment of cellular restoration which is prognostic index of fibrous remodeling development.
In view of the proved fact of influence of excitotoxicity on progressive course of dyscirculatory encephalopa- thy, clinical, neurophysiological investigation of the patients in dynamics of treatment with cytoflavin and cortexin. The positive clinical dynamics and partial normalization of electroencephalographic patterns were found out. Correlation analysis have proved ambiguous connection between clinical signs, focal changes in brain and therapeutic effectiveness. Results of investigation have proved the appropriateness of dyscirculatory encephalopathy therapy with cytoflavin and cortexin with the aim of inhibition of excitotoxicity. Multiplevalued connection between obtained parameters allow to assume the necessity of future investigations on this problem.
Cerebral transtentorial herniation determinates the course of progressive focal supratentorial impairments and acute hemorrhagic hemispheric stroke. Clinical and pathomorphological comparisons were done in patients with acute hemorrhagic hemispheric stroke. The following predictors of hemorrhagic hemispheric stroke lethal outcome were verified: 1) acute onset of coma in association with oculomotor dysfunctions; 2) focal hemiplegia in association with peduncular hemiplegia and paratonic rigiditydue due to compression of ipsilateral peduncle; 3) quick addition of vital disorders due to hemorrhagies into tectorium and it’s necrobiosis; 4) lethal outcome caused by herniation into occipital foramen due to necrobiosis of neurons in caudal part of brain stem tectorium.
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