9This study assessed and optimized an in vivo method to evaluate the levels of 10 susceptibility/resistance in fruit from the EEAD-CSIC peach germplasm to an isolate of 11 Monilinia laxa (Aderhold & Ruhland) Honey from peach. A total of four commercial 12 cultivars and six genotypes, descendants of three families, of peach [Prunus persica (L.) 13 88inoculation. 89Using the available methods, longer periods are required for obtaining the source of 90 inoculum and the amount and purity of inoculum are not assured. Moreover, an easy 91 5 inoculation system would help to screen large populations and controlled environment 92 would be preferable to avoid cross contamination. We have modified the existing protocols 93 by shortening the inoculum production and also ensuring the accurate supply of inoculum. 94The objective of this study, therefore, was to have an evaluation method, by optimizing the 95 available protocols, to screen tolerance to brown rot by Monilinia spp. in peach germplasm. 96 We have evaluated brown rot tolerance on Monilinia laxa ex situ inoculated peach fruit by 97 measuring brown rot incidence (%), lesion diameter (mm) and colonization extent (mm). In 98 the Spanish peach collection at the EEAD-CSIC, screening for resistance to brown rot has 99 shown that local germplasm accessions are susceptible although slight differences can be 100 found.
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