BackgroundA single-tier immunoassay using the C6 peptide of VlsE (C6) from Borrelia burgdorferi sensu stricto (Bb) has been proposed as a potential alternative to conventional two-tier testing for the serologic diagnosis of Lyme disease in the United States and Europe.ObjectiveTo evaluate the performance of C6 peptide based multiplex Phosphorescence Analysis (PHOSPHAN) for the serologic confirmation of Lyme borreliosis (LB) in Russian patients.MethodsSerum samples (n = 351) were collected from 146 patients with erythema migrans (EM); samples from 131 of these patients were taken several times prior to treatment and at different stages of recovery. The control group consisted of 197 healthy blood donors and 31 patients with other diseases, all from the same highly endemic region of Russia. All samples were analyzed by PHOSPHAN for IgM and IgG to Bb C6, recombinant OspC and VlsE proteins, and C6 peptides from B. garinii and B. afzelii.ResultsIgM and IgG to Bb C6 were identified in 43 and 95 out of 131 patients (32.8 and 72.5%, respectively); seroconversion of IgM antibodies was observed in about half of the patients (51.2%), and of IgG antibodies, in almost all of them (88.4%). Additional detection of OspC-IgM and VlsE-IgM or IgG to C6 from B. garinii or B. afzelii did not contribute significantly to the overall sensitivity of the multiplex immunoassay.ConclusionsThe multiplex phosphorescence immunoassay is a promising method for simultaneously revealing the spectrum of antibodies to several Borrelia antigens. Detection of IgM and IgG to Bb C6 in the sera of EM patients provides effective serologic confirmation of LB and, with high probability, indicates an active infection process.
The etiological structure of infections transmitted by Ixodes ticks (ITIT) was established in 467 (out of 522) patients of the Perm Regional Hospital for Infectious Diseases with the help of Laboratory Techniques Set, which included ELISA, PCR and PHOSPHAN М. There was stated that ITIT occurs most frequently - 235 (45, 0%) patients, tick borne encephalitis (TBE) -more rarely: 54 (10.4%), human granulocytic anaplasmosis (HGA) - 26 (5.0%) and human monocytic ehrlichiosis (HME) - 9 (1.7%). Various mixt-infections were detected in 143 patients (27.4%). In 55 patients (10.5%) the etiology of the disease remained to be unexplained. The clinical course of ITIT caused by genospecies B. afzelii and B. garinii is described.
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