No abstract

Ðåçþìå.  ñòàòüå ïðèâåäåíà èíôîðìàöèÿ î ðàçðàáîòêå è âíåäðåíèþ â ïðàêòèêó âåòåðèíàðíûõ ëàáîðàòîðèé ñîâðåìåííûõ èììóíîäèàãíîñòè÷åñêèõ ìåòîäîâ, â òîì ÷èñëå èììóíîôåðìåíòíîãî (ÈÔÀ) è èììóíîõðîìàòîãðàôè÷åñêîãî (ÈÕÌ). Ïðåäñòàâëåíû äàííûå î òåíäåíöèÿõ â ðàçâèòèè

Ðåçþìå.  ðåçóëüòàòå ïðîâåäåííîé ýêñïåðèìåíòàëüíîé ðàáîòû, ðàçðàáîòàí ìåòîä âûÿâëåíèÿ âîçáóäèòåëÿ ýíçîîòè÷åñêîé ïíåâìîíèè ñâèíåé (Mycoplasma hyopneumoniae) íà îñíîâå ïîìîùüþ ïîëèìåðàçíîé öåïíîé ðåàêöèè (ÏÖÐ), ñïåöèôè÷íîé ê êîíñåðâàòèâíûì ó÷àñòêàì ãåíîâ âèäîñïåöèôè÷íûõ áåëêîâ. Èññëåäîâàëèñü ïðîáû ïàòìàòåðèàëà îò aeèâîòíûõ ñ êëèíè÷åñêèìè ïðèçíàêàìè ðåñïèðàòîðíîãî ñèìïòîìîêîìïëåêñà, ïîëó÷åííûå èç ðàç-ëè÷íûõ ñâèíîâîä÷åñêèõ õîçÿéñòâ Ðîññèè. Èññëåäîâàëè ïðîáû êðîâè, ëåãî÷íûõ ëèìôîóçëîâ, aeèäêîñòè èç ãðóäíîé ïîëîñòè, ôðàãìåíòû ëåãêèõ ñâèíåé. Ïðîâîäèëè àíàëèç íóêëåîòèäíîé ïîñëåäîâàòåëüíîñòè ôðàãìåíòîâ ãåíîìà âûäåëåííûõ èçîëÿòîâ Mycoplasma hyopneumoniae. Summary. Detection method of Mycoplasma hyopneumoniae, causative agent of enzootic pneumonia of swine, was developed based on polymerase chain reaction using the genes of specie-specific proteins. The biologic probes were used from pathologic tissues from animals with clinical respiratory manifestations. These probes were collected from swine farms located at different geographical parts of Russia. Blood samples, lung lymph nodes, internal fluids, fragments of lungs were analyzed. The isolates of Mycoplasma hyopneumoniae were partially sequenced. Ââåäåíèå Ýíçîîòè÷åñêàÿ (ìèêîïëàçìåííàÿ) ïíåâìîíèÿ ñâèíåéèíôåêöèîííàÿ õðîíè÷åñêàÿ ýíçîîòè÷åñêàÿ áîëåçíü ñâèíåé âñåõ âîçðàñòîâ, ïðîÿâëÿþùàÿñÿ ðåìèòòèðóþùåé ëèõîðàäêîé, êàòàðàëüíîé áðîíõîïíåâìîíèåé, ñóõèì êàøëåì. Áîëåçíü âûçûâàåò çíà÷èòåëüíûå ýêîíîìè÷åñêèå ïîòåðè âñëåäñòâèå çàìåäëåíèÿ ðîñòà è ðàçâèòèÿ ïîðîñÿò èç-çà ïëîõîé óñâîÿåìîñòè êîðìà (çàðàaeåííûå aeèâîòíûå ðàñõîäóþò íà åäèíèöó ïðèðîñòà aeèâîé ìàññû íà 18-20% êîðìîâ áîëüøå, ÷åì çäîðîâûå). Ïîêàçàòåëè ñìåðòíîñòè ñîñòàâëÿþò îò 2 äî 15%, çàáîëåâàåìîñòü 38-100% [10].