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In this work the correlation of the neutron radiation damage and tungsten (W) microstructure is investigated. Further, the modification of the structure as a result of the neutron irradiation is assessed and its effect on the mechanical properties is determined. Forged bar (ITER grade), cold rolled sheet, and single crystalline tungsten materials were neutron irradiated at 600 °C to a damage of 0.12 displacements per atom. Neutron irradiation results in the formation of voids of almost the same size (larger than 1 nm) and dislocations detected by positron annihilation lifetime spectroscopy. All W grades have similar total dislocation densities, in the range of (1.6-2.4)×10 14 m −2 , as determined by electrical resistivity measurements. After irradiation the hardness of all tungsten grades increases and the largest increase is that of the single crystal (47%), whereas the smallest that of the sheet (13%). Increase in the yield strength, correlated to the increase of the hardness, is also found. The largest increase is observed for the single crystal (25%) and the smallest for the sheet (6%). The different degrees of hardening and strengthening indicate that the microstructure of the different tungsten grades has a significant influence on their neutron radiation damage resistance.