A huge group of natural antimicrobial compounds are active against a large spectrum of bacterial strains causing infectious threat. The present study was conducted to investigate the crude extracts of antimicrobial protein and peptide efficacy from six medicinal plant seeds. Extraction was carried out in Sodium phosphate citrate buffer, and Sodium acetate buffer using different pH. Antimicrobial activities of these plants were determined by the microbiological technique using Agar well diffusion Assay. Extremely strong activity was observed in the seed extracts of Allium ascolinicum extracted in sodium phosphate citrate buffer at pH (5.8) against Proteus vulgaris, Escherichia coli and Staphylococcus aureus with zone of inhibition 17 mm, 17 mm and 15 mm and Rumex vesicarius at pH (7.6), Ammi majus at pH (6.8), Cichorium intybus at pH (7.4) and Cucumis sativus at pH (7.8) also showed better sensitivity against the bacterial strains with zone of inhibition ranges 16-10 mm and some of the strains were found to be resistant. Antibacterial activity pattern of different plant extracts prepared in sodium acetate buffer pH (6.5), among all the plant seed extracts used Foeniculum vulgare had shown good inhibition in all the bacterial strains used, with zone of inhibition ranges 11-12.5 mm, The extracts of C. intybus and C. sativus were found to be effective with zone of inhibition 11-6 mm and some of the strains were found to be resistant. Most of the strains found to have shown better sensitivity compared with the standard antibiotic Chloramphenicol (25 mcg). Our results showed that the plants used for our study are the richest source for antimicrobial proteins and peptides and they may be used for industrial extraction and isolation of antimicrobial compounds which may find a place in medicine industry as constituents of antibiotics.
Herbal medications have been used for relief of symptoms of disease. Regardless of the great advances observed in current medicine in recent decades, plants still make a significant contribution to health care. An alarming increase in bacterial strains resistant to a number of antimicrobial agents demands that a renewed effort be made to seek antibacterial agents effective against pathogenic bacteria resistant to or less sensitive to current antibiotics. Anti-bacterial activity of stem bark was tested against pathogenic and using various solvent extracts. The in vitro anti-bacterial activity was performed by agar well diffusion method and the results were expressed as the average diameter of zone of inhibition of bacterial growth around the well. The ethanol and methanol extracts showed better anti-bacterial activity with zone of inhibition (20-25 mm) when compared with other tested extracts and standard antibiotic Erythromycin (15 mcg) with zone of inhibition (13-14 mm). Using Fisher's exact test of significance difference was found between two Salmonella strains sensitivity patterns against tested extracts ( ⩽ 0.035). Extracts of stem bark also exhibited significant antioxidant activity, thus establishing the extracts as an antioxidant. The results obtained in this study give some scientific support to the stem bark for further investigation of compounds and in future could be used as drug.
Background:With global realization that use of synthetic drugs is not safe on the long run, the medical fraternity at large is looking at alternatives from natural sources to combat diseases particularly those wherein conventional modern system of medicine has little to offer. On one hand, this realization has increased demand for herbal drugs and on the other hand need for quality standardization of these drugs has increased. Central Research Institute of Unani Medicine, Hyderabad, being engaged in multidisciplinary research in Unani Medicine, has taken up standardization of herbomineral drugs used in this system of medicine. One such drug “Qurs-e-Luk” that is widely used by the practitioners of this system in mobilizing and reducing body fat has been utilized for standardization by modern techniques, so as to ascertain its quality.Methods:The parameters which were carried out are pharmacognostic studies, physicochemical parameters, high-performance thin layer chromatography, microbial load, aflatoxins, heavy metals, and pesticidal residues revealing specific identities for the particular drug and to evaluate pharmacopoeial standards.Results and Conclusion:Results suggest that the drug is safe for therapeutic use and its batch to batch identification for quality control is possible on the basis of present study.
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