Fig. 3 ER beta recruits corepressors to the Sp-1 site in the ER alpha gene promoter. a Nuclear extracts from MCF-7 cells transfected with either empty vector or ER beta expression plasmid were incubated with a double-stranded Sp-1 specific sequence probe labeled with [c 32 P] ATP and subjected to elettrophoresis in a 6 % polyacrylamide gel (lanes 1 and 4). Competition experiments were performed adding as competitor a 100-fold molar excess of unlabeled probe (lanes 2 and 11) or a 100-fold molar excess of unlabeled oligonucleotide containing a mutated Sp-1 motif (lane 3). Nuclear extracts from MCF-7 over-expressing ER beta were incubated with anti-NCoR
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