Introduction. Vitamin D3 possesses antioxidant, anti-inflammatory, immunomodulatory, and other properties, has been shown to be effective in some autoimmune diseases, which is a prerequisite for studying its effect, when applied locally, on the clinical status and morphology of the site of injury in ulcerative colitis (UC).The aims was to study the effect of vitamin D3 in the composition of original rectal suppositories on the clinical status and morphology of the lesion of colon in experimental UC.Materials and methods. UC was modeled by two-stage administration of oxazolone. Rectal suppositories were prepared on the basis of a 10% aqueous solution of vitamin D3. The clinical status was assessed using the Disease activity index (DAI) scale. In the area of colon damage, the number of neutrophils, lymphocytes, eosinophils, histiocytes, plasmocytes and fibroblasts was examined per mm² d the tissue damage index (TDI) was calculated.Results and Discussion. In experimental UC, DAI increase an ulcerative defect is recorded in the colon, the number of neutrophils, lymphocytes, eosinophils, plasma cells, histiocytes, fibroblasts, TDI increases. The use of original rectal suppositories with vitamin D3 in experimental UC leads to a decrease in DAI, the size of the ulcer and TDI, a decrease in the infiltration of the intestinal wall by neutrophils, lymphocytes, eosinophils and plasma cells, an increase in the infiltration of histiocytes, fibroblasts.Conclusions. In experimental oxazolone-induced colitis, the clinical picture and morphology of the injury site characteristic of UC are recorded. The use of original rectal suppositories with vitamin D3 a total dose of 18,000 IU leads to a decrease in the severity of clinical signs and a decrease in the representation in the colon wall of cells involved in tissue destruction, an increase in the representation of cells mediating repair, which was reflected in a decrease in the area of the ulcer and tissue damage index , the severity of clinical signs according to DAI weakens as lymphocytes and plasma cells decrease in the area of damage to the colon, decrease in the size of the ulcer and decrease in TDI, increase in histiocytes and fibroblasts.
Introduction. At the moment in the Russian Federation there are no dosage forms with vitamin D3, allowing effective delivery and local effect on the focus of inflammation and the damaged area of the large intestine in inflammatory bowel disease (IBD). Among such dosage forms rectal suppositories are of the greatest interest. The aim of the study was to carry out a comparative analysis of the effectiveness of local administration of vitamin D3 and 5-aminosalicylic acid in experimental colitis. Materials and methods. Experimental colitis (EC) was modeled with oxazolone solution. Suppositories with vitamin D3 and with 5-ASA were applied per rectum every 12 h. Clinical status (DAI), morphometry, colon tissue injury index (TDI), myeloperoxidase (MPO) and TNF-α expression in the lesion were assessed. Results. In EC, DAI is increases, an ulcerative defect is fixed in the lesion of the colon, TDI, neutrophils (NF), lymphocytes (LC), eosinophils (EF), histiocytes (HC), plasma cells (PC), fibroblasts (FB), MPO and TNF-α expression are increased. Vitamin D3 administration reduces DAI, ulcer defect, TDI, MPO and TNF-α expression, the number of NF, EF, LC and PCs, and increases the number of GCs and FBs. Comparison of vitamin D3 and 5-ASA administration revealed comparable efficacy against DAI. Morphometric evaluation of colorectal lesions showed that under the conditions of vitamin D3 administration, in contrast to 5-ASC, less infiltration, edema, signs of healing and repair of ulcerous defects were fixed earlier in EC; MPO expression increased on the 6th day, TNF-α expression on the 4th day. The TDI index on the 4th and 6th days of EC decreased equally under the conditions of vitamin D3 and 5-ASC application. Discussion. The reduction of clinical severity and morphological signs of damage in the large intestine wall at EC against the background of using rectal suppositories with vitamin D3 could be due to pleiotropic effects of vitamin D3. Conclusion. The effect of vitamin D3 in original rectal suppositories is comparable with local application of 5-ASC at EC, it reduces severity of clinical signs, representation of cells involved in tissue destruction, TNF-α and MPO expression in the colon wall and increases representation of cells mediating reparation.
Increased incidence of ulcerative colitis (UC) is a prerequisite for searching new therapeutic approaches, primarily with an opportunity of site-directed impact on the colon lesion. UC pathogenesis is associated with dysregulated immune response, and limited effectiveness of basic therapy for the disorder. Vitamin D3 exhibits antioxidant, anti-inflammatory, immunomodulatory and other properties, it has been shown to be effective in some autoimmune diseases, thus prompting us to study its effect on immune status in UC. We aimed for studying the effect of vitamin D3, as a component of original rectal suppositories, upon clinical course and indexes of immune status in experimental UC. UC in rats was modeled with 3% oxazolone solution. The vitamin D3-containing suppositories (1500 IU) weighing 300 mg were administered per rectum every 12 hours for 6 days. On days 2, 4 and 6 of UC, the clinical features were assessed as well as blood leukocyte counts, numbers of CD3+, CD45RA+; absorbing and NBT-reducing abilities of blood neutrophils were determined; IgM, IgG, IL-6 and IL-8 concentrations in serum were also studied.The DAI index increased in non-treated UC, along with raised neutrophil numbers in blood, their absorption and NBT-reducing activity was also increased, the total number of lymphocytes, including CD3+, CD45RA+ became higher, serum concentrations of IgM, IgG, IL-6, IL-8 increased. Local use of vitamin D3 in UC reduces DAI parameters, causes decrease in blood neutrophil counts, reducing and partially restoring absorptive and NBT-reducing abilities of neutrophils, decline of total lymphocyte counts in blood, partially restoring the CD3+ and CD45RA+ numbers, causing decline and partial restoration of serum IgM, IgG, IL-6, IL-8 concentrations. An association between clinical signs and indexes of immune status in UC was established under the conditions of vitamin D3 use. Conclusions: The protective effect of vitamin D3 in UC can be mediated by its antioxidant effect, changes in production of immunoregulatory cytokines, modulation of Th1-, Th2-, Th17-dependent reactions and Treg activity, being a pre-requisite for further studies to clarify the mechanism of vitamin D3 immunotropic action in UC,with an opportunity of using it in clinical practice.
Анкилозирующий спондилит (АС) -хроническое вос-палительное заболевание из группы спондилоартритов (СпА), характеризующееся обязательным поражением кре-стцово-подвздошного сочленения (КПС) и/или позвоноч-ника с потенциальным исходом в анкилоз, с частым вовле-чением в патологический процесс энтезисов и перифериче-ских суставов [1].Целью лечения пациента со СпА, включая АС, явля-ется достижение ремиссии или, в качестве альтернатив-ной цели, низкой активности заболевания с улучшением качества жизни и замедлением прогрессирования [2]. До-стижение клинико-лабораторной ремиссии и ремиссии по данным магнитно-резонансной томографии (МРТ) возможно на фоне длительной и зачастую дорогостоящей
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