No abstract

Cell damage by low-power 365-nm radiation of a 50-W high-pressure mercury microscopy lamp was studied. Exposure of Chinese hamster ovary (CHO) cells to ultraviolet-A (UVA) radiation Ͼ10 kJ/m 2 resulted in significant modifications of nicotinamide adenine dinucleotide (NADH) attributed autofluorescence and inhibition of cell division. Single-cell gel electrophoresis (comet assay) revealed UVA-induced single-strand DNA breaks. According to these results, UVA excitation radiation in fluorescence microscopy may damage cells. This has to be considered in vital cell microscopy, e.g., in calcium measurements.

No abstract

No abstract

No abstract