Biocidal products (disinfectants) are intensively used in nursing homes to control and prevent the spread of microorganisms and healthcare associated infections. We checked the hygiene of the surfaces intended for multiple use and the microbial population present on these surfaces. In three different areas, we noticed the working protocol and the biocidal products in use. The surfaces of the devices were sampled with swabbing before use, after use and after disinfection. Identification and quantification of microorganisms was performed using classical culture methods and MALDI-TOF method. The results showed that some devices were contaminated before use. Of particular concern is the fact that microorganisms remain on the devices even after disinfection in concentrations from 1 to 300 cfu/mL. In complex microbial communities on surfaces and with intensive use of disinfectants, the acquired bacterial resistance to disinfectants can develop, which can consequently lead to increased bacterial resistance to antibiotics.
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